- Cell Line: HCC1143 BL
- Synonyms:
- HCC1143 BL
- HCC 1143 BL
- HCC1143 BL
- HCC 1143 BL
- HCC-1143BL
- HCC1143-BL
- HCC1143BL
- Hamon Cancer Center 1143 BL
- Culture Type: Cell line
- Keywords: Human B lymphoblast EBV-transformed cell line
- Description: HCC1143 BL is a normal B lymphoblast cell that was isolated from the peripheral blood of a 52-year-old, white female patient with ductal carcinoma of the breast. Subsequently, cells were immortalized by transformation with EBV.
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age: 52 years
- Ethnicity: White
- Gender: Female
- Disease: Normal
- Tissue (origin): Peripheral blood
- Year of origin:-
- Organism:
- Cell line features:
- Morphology: lymphoblast
- Cell Type: B lymphoblast
- Growth mode: Suspension, multicellular aggregates
- Life span: Infinite (continuous culture)
- Karyotype:-
- Doubling time:-
- Sequence variations (gene mutations/deletions/fusions/translocations):–
- Gene expression:-
- Morphology: lymphoblast
- Handling and maintenance:
- Biosafety level: BSL 2 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: RPMI-1640 + 10% FBS
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Dilution
- Maintain cultures at cell concentrations between 5 x 105 and 2 x 106 viable cells/mL.
- Cryopreservation:
- Freezing medium: Complete growth medium + 5% DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density): 2 – 4 x 106 viable cells/ml
- Volume per vial: 1 ml
- Freezing medium: Complete growth medium + 5% DMSO
- Applications:
- Cancer research
- 3D cell culture
- Availability
- American Type Culture Collection (ATCC): Product link
- References
- https://www.atcc.org/products/crl-2362
- https://www.cellosaurus.org/CVCL_1246
- Gazdar et al., 1998. Characterization of paired tumor and non-tumor cell lines established from patients with breast cancer. Int. J. Cancer 78: 766-774, 1998. PMID-9833771; Full Text: Wiley (Download PDF)
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
- EBV (Epstein-Barr virus)
- Article Authenticity Index *
- Please let us know if you find any mistakes. Your comments are welcome in the comment box.
