- Cell Line: HCC1599 BL
- Synonyms:
- HCC1599BL
- HCC-1599BL
- HCC1599-BL
- Hamon Cancer Center 1599 BL
- Culture Type: Cell line
- Keywords: Epstein-Barr virus (EBV) transformed normal B lymphoblast cells
- Description: Established from the peripheral blood of a 44-year-old white woman with ductal breast carcinoma
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age: 44 years
- Ethnicity: White
- Gender: Female
- Tissue (origin): Peripheral blood
- Disease: Normal
- Disease subtype: Matched Normal (an autologous carcinoma cell line HCC1599 is available)
- Year of origin: 1995
- Organism:
- Cell line features:
- Morphology: Round to polymorphic cells
- Cell Type: B lymphoblast
- Growth mode: Suspension, single cells and multicellular aggregates
- Life span: Infinite (continuous culture)
- Karyotype:-
- Doubling time: ≈ 48-72 hours (DSMZ)
- Immortalization method: Epstein-Barr virus (EBV) transformed
- Gene expression:-
- Sequence variations (gene mutations/deletions/fusions/translocations):
- For information, please visit
- Morphology: Round to polymorphic cells
- Handling and maintenance:
- Biosafety level: BSL 2 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: RPMI-1640 + 10% FBS
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Dilution
- Maintain cultures at cell concentrations between 5 x 105 and 2 x 106 viable cells/mL.
- Medium Renewal: Every 3 to 4 days (depending on cell density)
- Cryopreservation:
- Applications:
- Immunology
- 3D cell culture
- Availability
- American Type Culture Collection (ATCC): Product link
- DSMZ-German Collection of Microorganisms and Cell Cultures GmbH: Product link
- References
- https://www.atcc.org/products/crl-2332
- https://www.dsmz.de/collection/catalogue/details/culture/ACC-894
- https://www.cellosaurus.org/CVCL_1257
- https://depmap.org/portal/cell_line/ACH-002374?tab=overview
- Gazdar et al., 1998. Characterization of paired tumor and non-tumor cell lines established from patients with breast cancer. Int. J. Cancer 78: 766-774, 1998. PMID-9833771; Full Text: Wiley (Download PDF)
- Images
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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