Description: HEK293-A cells (subclone of HEK-293) stably expressing GFP-tagged human WIPI1
- Synonyms: HEK293-A GFP-WIPI1
- Keywords: HEK293-A cells (a subclone of HEK-293) stably expressing GFP-tagged human WIPI1
- Culture Type: Cell line
- Origin/Source:
- Organism:
- Species: Human
- Age: Fetus
- Tissue of Origin: Embryonic Kidney
- Organism:
- Cell line features:
- Morphology: Adherent polygonal epithelium
- Cell Type: Epithelial
- Growth Mode: Adherent
- Applications:
- Useful for high-throughput microscopy screens
- Apoptosis and Programmed Cell Death
- Cancer
- Cell Signaling & Signal Transduction
- Cellular Transport
- Drug Discovery & Development
- Metabolism
- Handling and maintenance
- Biosafety level: BSL 2 (It may differ in your country, follow the guidelines of your institute/country)
- GMO Status: GMO1
- Complete culture medium:
- DMEM, high glucose + 2mM Glutamine + 10% FBS + G418 (400 µg/ml)
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 10% CO₂, 90% Air (culturecollections)
- Subculturing:
- Cell dissociation procedure: 0.05% Trypsin/EDTA or Trypsin
- Harvesting time: 80% confluence culture
- Subcultivation Ratio:
- Medium Renewal:
- Seeding densities: 2×10⁴ cells/cm²
- Cryopreservation
- Growth medium + 10% DMSO
- Cryopreservation temperature: Liquid nitrogen gas phase or -150°C freezer
- Availability:
- Culture Collections (UK Health Security Agency): Product link
- References:
- https://www.culturecollections.org.uk/nop/product/hek293-a-gfp-wipi1
- Dooley et al., 2014. WIPI2 links LC3 conjugation with PI3P, autophagosome formation, and pathogen clearance by recruiting Atg12-5-16L1. Mol Cell. 55(2), 238-252. PMID-24954904; Full-Text Links: Cell, PMC4104028
- Abbreviations
- DMEM (Dulbecco’s Modified Eagle’s Medium)
- FBS (Foetal Bovine Serum)
- GMO1 (Genetically Modified Organism Class 1)
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