- Cell Line: N18TG2
- Synonyms:
- N18Tg2
- N18 tg 2
- Culture Type: Cell line
- Keywords: Mouse Neuroblastoma Cell Line
- Description: 6-thioguanine resistant mutant of N18 cell line. N18 was established from a mouse of strain A/J with neuroblastoma C1300.
- Origin:
- Organism:
- Species: Human (Mus musculus)
- Breed: A/J
- Gender: Male
- Tissue (origin): Peripheral nervous system/Neuroblastoma
- Disease: Neuroblastoma
- Primary/Metastasis:-
- Collection site:-
- Year of origin:-
- Organism:
- Cell line features:
- Cell Type: Neuronal (Neural crest derived neuroblast)
- Morphology: round, single, slightly adherent cells, occasionally with short processes
- Growth mode: Adherent
- Life span: Infinite (continuous culture)
- Karyotype: Aneuploid
- Doubling time: ~40 hours
- Microsatellite instability:-
- Special features:-
- Product: Expresses acetylcholinesterase
- Soft agar colony formation assay:-
- Tumorigenicity: Yes, in BALB/c nude mice
- Gene expression:
- Sequence variations:
- Cell Type: Neuronal (Neural crest derived neuroblast)
- Handling and maintenance:
- Biosafety level: BSL 1 (DSMZ) (It may differ in your country, follow the guidelines of your institute/country)
- Hazard Group (ACDP): Hazard Group (ACDP) 2
- Complete culture medium: DMEM + 2 mM glutamine + 10% FBS + 100 microM 6-thioguanine
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Enzymatic dissociation
- Cell dissociation solution: Trypsin-EDTA solution
- Harvesting time:-
- Seeding density: 2 to 4 x 104 viable cells/cm²
- Split ratio: 1:3 to 1:6
- Split frequency: twice a week
- Medium Renewal: Every 2 to 3 days
- Subculturing method: Enzymatic dissociation
- Cryopreservation:
- Freezing medium:
- Complete growth medium + 20% FBS + 10% DMSO
- Complete growth medium + 20% FBS + 10% DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density): 1-2 x 106 cells/ampoule
- Volume per vial: 1 ml
- Freezing medium:
- Applications:
- Used in the generation of cell hybrids
- Used in the generation of cell hybrids
- Availability:
- UK Health Security Agency Culture Collections: Product link
- DSMZ-German Collection of Microorganisms and Cell Cultures GmbH: Product link
- Sigma-Aldrich: Product link
- References
- Images:
- N18TG2 cell line image linkm1 (Source: DSMZ)
- N18TG2 cell line image link 2 (Source: DSMZ)
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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