- Cell Line: NCI-H716
- Synonyms:
- NCI-H716
- NCIH716
- H716
- H-716
- Culture Type: Cell line
- Keywords: Human Colorectal Adenocarcinoma Cell Line
- Description: Established from the cecum of a Caucasian, 33-year-old, male patient with Colorectal adenocarcinoma
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age: 33 years
- Ethnicity: Caucasian
- Gender: Male
- Smoking habit:-
- Tissue (origin): Large intestine, Cecum
- Disease: Colorectal adenocarcinoma
- Primary/Metastasis: Metastatic
- Collection site: Ascites
- Year of origin: 1984
- Organism:
- Cell line features:
- Cell Type:-
- Morphology: Lymphoblast-like
- Growth mode: Suspension
- Life span: Infinite (continuous culture)
- Karyotype: Modal number = 61, range = 55 to 64
- Doubling time: 50 hours
- Microsatellite instability: Stable
- Special features:-
- Product: Secretes high level of glucagon-like peptide 1 (GLP-1)
- Soft agar colony formation assay:-
- Tumorigenicity: Yes, in nude mice
- Gene expression:
- Sequence variations:
- TP53 (E224D mutation, Likely Oncogenic, Likely Loss-of-function, Homozygous)
- For information, please visit
- https://depmap.org/portal/cell_line/ACH-000491?tab=mutations
- https://depmap.org/portal/cell_line/ACH-000491?tab=fusions
- Cell Type:-
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: RPMI-1640 Medium + 10% fetal bovine serum
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Dilution
- Cell dissociation solution: NA
- Harvesting time:-
- Seeding density: 4 to 8 x 105 viable cells/mL
- Recommended split ratio: 1:3 to 1:6
- Medium Renewal: Every 2 to 3 days
- Subculturing method: Dilution
- Cryopreservation:
- Freezing medium:
- Complete growth medium + 5% (v/v) DMSO
- Complete growth medium + 5% (v/v) DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial: 1 ml
- Freezing medium:
- Applications:
- 3D cell culture
- Cancer research
- Availability:
- American Type Culture Collection (ATCC): Product link
- Cytion: Product link
- References
- Images:
- NCI-H716 cell line image link (Source: ATCC)
- CI-H716 cell line image link 1 (Source: Cytion)
- NCI-H716 cell line image link 2 (Source: Cytion)
- NCI-H716 cell line image link 3 (Source: Cytion)
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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