- Cell Line: NCI-H196
- Synonyms:
- NCI-H196
- NCIH196
- H196
- H-196
- Culture Type: Cell line
- Keywords: Human Lung Carcinoma Cell Line
- Description: Established from the lungs of a 68-year-old Caucasian male with stage E carcinoma
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age: 68 years
- Ethnicity: Caucasian
- Gender: Male
- Smoking habit:-
- Tissue (origin): Lung
- Disease: Small cell lung cancer, Stage E
- Primary/Metastasis: Metastatic
- Collection site: Pleural effusion
- Year of origin: 1979
- Organism:
- Cell line features:
- Cell Type:-
- Morphology:-
- Growth mode: Adherent
- Life span: Infinite (continuous culture)
- Karyotype:
- Doubling time: 64 hours
- Microsatellite instability: Stable
- Special features:-
- Product:-
- Soft agar colony formation assay:-
- Tumorigenicity:-
- Gene expression:
- Sequence variations:
- PTEN (Y138C mutation, Likely Oncogenic Likely Loss-of-function)
- TP53 (R175H mutation, Oncogenic, Loss-of-function)
- For information, please visit
- https://depmap.org/portal/cell_line/ACH-000752?tab=mutations
- https://depmap.org/portal/cell_line/ACH-000752?tab=fusions
- Cell Type:-
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: RPMI-1640 Medium + 10% fetal bovine serum
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Enzymatic dissociation
- Cell dissociation solution: 0.25% trypsin, 0.03% EDTA solution
- Harvesting time:-
- Seeding density:-
- Recommended split ratio: 1:4 to 1:6
- Medium Renewal: Every 2 to 3 days
- Subculturing method: Enzymatic dissociation
- Cryopreservation:
- Freezing medium:
- Complete growth medium + 5% DMSO
- Complete growth medium + 5% DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial: 1 ml
- Freezing medium:
- Applications:
- Cancer research
- 3D cell culture
- Cancer research
- Availability:
- American Type Culture Collection (ATCC): Product link
- Cytion: Product link
- References
- Images:
- NCI-H196 cell line image link (Source: DepMap)
- NCI-H196 cell line image link 1 (Source: Cytion)
- NCI-H196 cell line image link 2 (Source: Cytion)
- NCI-H196 cell line image link 3 (Source: Cytion)
- NCI-H196 cell line image link 4 (Source: Cytion)
- NCI-H196 cell line image link 5 (Source: Cytion)
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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- Please let us know if you find any mistakes. Your comments are welcome in the comment box.
