- Cell Line: NCI-H28
- Synonyms:
- NCI-H28
- NCIH28
- H28
- H-28
- Culture Type: Cell line
- Keywords: Human Lung Mesothelioma Cell Line
- Description: Established from the lungs of a 48-year-old, Caucasian male with stage 4 mesothelioma
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age: 48 years
- Ethnicity: Caucasian
- Gender: Male
- Smoking habit: Smoker
- Tissue (origin): Lung
- Disease: Mesothelioma; Stage 4
- Primary/Metastasis: Metastatic
- Collection site: Pleural effusion
- Year of origin: 1976
- Organism:
- Cell line features:
- Cell Type:-
- Morphology:-
- Growth mode: Adherent
- Life span: Infinite (continuous culture)
- Karyotype: t(3p;14q)
- Doubling time:
- Microsatellite instability: Instable
- Special features:-
- Product:-
- Soft agar colony formation assay:-
- Tumorigenicity:-
- Gene expression:
- Sequence variations:
- VHL (L89H mutation, Likely Oncogenic Unknown, Homozygous)
- For information, please visit
- https://depmap.org/portal/cell_line/ACH-000648?tab=mutations
- https://depmap.org/portal/cell_line/ACH-000648?tab=fusions
- Cell Type:-
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: RPMI-1640 Medium + 10% fetal bovine serum
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Enzymatic dissociation
- Cell dissociation solution: 0.25% (w/v) Trypsin- 0.53 mM EDTA solution
- Harvesting time:-
- Seeding density:-
- Recommended split ratio: 1:3 to 1:6
- Medium Renewal: Every 2 to 3 days
- Subculturing method: Enzymatic dissociation
- Cryopreservation:
- Freezing medium:
- Complete growth medium + 5% DMSO
- Complete growth medium + 5% DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial: 1 ml
- Freezing medium:
- Applications:
- Cancer research
- 3D cell culture
- Cancer research
- Availability:
- American Type Culture Collection (ATCC): Product link
- References
- Images:
- NCI-H28 cell line image link (Source: DepMap)
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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- Please let us know if you find any mistakes. Your comments are welcome in the comment box.
