- Cell Line: NCI-H2029
- Synonyms:
- NCI-H2029
- NCIH2029
- H2029
- H-2029
- Culture Type: Cell line
- Keywords: Human Lung Carcinoma Cell Line
- Description: Established from the lungs of a 69-year-old, Caucasian, female patient with small cell lung cancer, stage E
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age: 69 years
- Ethnicity: Caucasian
- Gender: Female
- Smoking habit: smoker
- Tissue (origin): Lung
- Disease: Small cell lung cancer; Stage E
- Primary/Metastasis: Metastatic (Lymph node)
- Year of origin: 1988
- Organism:
- Cell line features:
- Cell Type:-
- Morphology:-
- Growth mode: Adherent
- Life span: Infinite (continuous culture)
- Karyotype:-
- Doubling time:-
- Microsatellite instability: Stable
- Special features:-
- Product:-
- Soft agar colony formation assay:-
- Tumorigenicity:-
- Gene expression:
- Sequence variations:
- TP53 (Y220D mutation, Likely Oncogenic, Likely Loss-of-function, Homozygous)
- For information, please visit
- https://depmap.org/portal/cell_line/ACH-000298?tab=mutations
- https://depmap.org/portal/cell_line/ACH-000298?tab=fusions
- https://depmap.org/portal/cell_line/ACH-000298?tab=translocations
- Cell Type:-
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: DMEM:F12 Medium supplemented with
- 0.005 mg/ml Insulin
- 0.01 mg/ml Transferrin
- 30nM Sodium selenite
- 10 nM Hydrocortisone
- 10 nM beta-estradiol
- extra 2mM L-glutamine (for final conc. of 4.5 mM)
- 5% fetal bovine serum
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Enzymatic dissociation
- Cell dissociation solution: 0.25% (w/v) Trypsin-0.53mM EDTA solution?
- Harvesting time:-
- Seeding density:-
- Recommended split ratio: 1:3 to 1:5
- Medium Renewal: Every 2 to 3 days
- Subculturing method: Enzymatic dissociation
- Cryopreservation:
- Freezing medium:
- Complete growth medium + 5% (v/v) FBS + 5 % (v/v) DMSO
- Complete growth medium + 5% (v/v) FBS + 5 % (v/v) DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial: 1 ml
- Freezing medium:
- Applications:
- Cancer research
- 3D cell culture
- Cancer research
- Availability:
- American Type Culture Collection (ATCC): Product link
- References
- Images:-
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
- Article Authenticity Index *
- Please let us know if you find any mistakes. Your comments are welcome in the comment box.
Was this post helpful?
Let us know if you liked the post. That’s the only way we can improve.
