- Cell Line: NCI-H2291
- Synonyms:
- NCI-H2291
- NCIH2291
- H2291
- H-2291
- Culture Type: Cell line
- Keywords: Human Lung Adenocarcinoma Cell line
- Description: Established from the metastatic tumor (Lymph node) of a caucasian, male patient with non-small cell lung cancer (adenocarcinoma).
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age:-
- Ethnicity: Caucasian
- Gender: Male
- Smoking habit: non-smoker
- Tissue (origin): Lung
- Disease: Non-small cell lung cancer
- Disease subtype: Lung Adenocarcinoma
- Molecular subtype:-
- Primary/Metastasis: Metastatic (Lymph node)
- Year of origin: 1990
- Organism:
- Cell line features:
- Cell Type:-
- Morphology: Epithelial-like
- Growth mode: Adherent
- Life span: Infinite (continuous culture)
- Karyotype:-
Doubling time:- - Microsatellite instability: Stable
- Special features:
- Product:-
- Soft agar colony formation assay:-
- Tumorigenicity:-
- Gene expression:
- Sequence variations:
- TP53 (G154V mutation, Likely Oncogenic, Likely Loss-of-function, Homozygous)
- KRAS (G12F mutation, Likely Oncogenic, Likely Gain-of-function, Homozygous)
- For information, please visit
- https://depmap.org/portal/cell_line/ACH-000718?tab=mutations
- https://depmap.org/portal/cell_line/ACH-000718?tab=fusions
- Cell Type:-
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: RPMI-1640 Medium + 10% FBS
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Enzymatic dissociation
- Cell dissociation solution: 0.25% (w/v) Trypsin-0.53 mM EDTA solution
- Harvesting time:-
- Seeding density:-
- Recommended split ratio: 1:3 to 1:4
- Medium Renewal: Every 2 to 3 days
- Cryopreservation:
- Freezing medium:
- Complete growth medium + 5% DMSO
- Complete growth medium + 5% DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial: 1 ml
- Freezing medium:
- Applications:
- Cancer research
- 3D cell culture
- Availability:
- American Type Culture Collection (ATCC): Product link
- American Type Culture Collection (ATCC): Product link
- References
- Images: NCI-H2291 cell Image link (Source: DepMap)
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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