- Cell Line: NCI-H661
- Synonyms:
- NCI-H661
- NCIH661
- H661
- H-661
- Culture Type: Cell line
- Keywords: Human Non-Small Cell Lung Cancer Cell line
- Description: Established from the metastatic tumor (Lymph node) of a 43-year-old, caucasian, male with non-small cell lung cancer.
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age: 43 years
- Ethnicity: Caucasian
- Gender: Male
- Smoking habit:-
- Tissue (origin): Lung
- Disease: Non-small cell lung cancer
- Disease subtype: Lung large cell carcinoma
- Molecular subtype:-
- Disease subtype: Lung large cell carcinoma
- Primary/Metastasis: Metastatic (Lymph node)
- Year of origin: 1982
- Organism:
- Cell line features:
- Cell Type:-
- Morphology: Epithelial-like
- Growth mode: Adherent
- Life span: Infinite (continuous culture)
- Karyotype: hyperhexaploid, modal number = 142, range = 130 to 153
- Doubling time: 61 hours
- Microsatellite instability: Stable
- Special features: Express easily detectable p53 mRNA at levels comparable to normal lung tissue
- Product:-
- Soft agar colony formation assay:-
- Tumorigenicity:-
- Gene expression:
- Sequence variations:
- TP53 (S215I mutation, Likely Oncogenic, Likely Loss-of-function, Heterozygous)
- For information, please visit
- https://depmap.org/portal/cell_line/ACH-000853?tab=mutations
- https://depmap.org/portal/cell_line/ACH-000853?tab=fusions
- Cell Type:-
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: RPMI-1640 Medium + 10% FBS
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Enzymatic dissociation
- Cell dissociation solution: 0.25% (w/v) Trypsin-0.53 mM EDTA solution
- Harvesting time:-
- Seeding density:-
- Recommended split ratio: 1:3 to 1:5
- Medium Renewal: 2 to 3 times per week
- Cryopreservation:
- Freezing medium:
- Complete growth medium + 5% DMSO
- Complete growth medium + 5% DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial: 1 ml
- Freezing medium:
- Applications:
- Cancer research
- 3D cell culture
- Availability:
- American Type Culture Collection (ATCC): Product link
- American Type Culture Collection (ATCC): Product link
- References
- Images:
- NCI-H661 cell Image link 1 (Source: cytion.com)
- NCI-H661 cell Image link 2 (Source: cytion.com)
- NCI-H661 cell Image link 3 (Source: cytion.com)
- NCI-H661 cell Image link 4 (Source: cytion.com)
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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- Please let us know if you find any mistakes. Your comments are welcome in the comment box.
