- Cell Line: NCI-H841
- Synonyms:
- NCI-H841
- NCIH841
- H841
- H-841
- Culture Type: Cell line
- Keywords: Human Small Cell Lung Cancer Cell line
- Description: Established from lungs of a 51-year-old, Caucasian male with Stage L carcinoma
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age: 51 years
- Ethnicity: Caucasian
- Gender: Male
- Smoking habit: smoker
- Tissue (origin): Lung
- Disease: Lung small cell carcinoma, Stage L
- Primary/Metastasis: Metastatic
- Year of origin: 1984
- Organism:
- Cell line features:
- Cell Type:-
- Morphology: Epithelial-like (adherent cells)
- Growth mode: Mixed: adherent and suspension
- Life span: Infinite (continuous culture)
- Karyotype: t(2p,3p), t(3q,9q)
Doubling time:- - Microsatellite instability: Stable
- Special features:
- Product:-
- Soft agar colony formation assay:-
- Tumorigenicity:-
- Gene expression:
- Sequence variations:
- TP53 (C242S mutation, Likely Oncogenic, Loss-of-function, Homozygous)
- For information, please visit
- https://depmap.org/portal/cell_line/ACH-000292?tab=mutations
- https://depmap.org/portal/cell_line/ACH-000292?tab=fusions
- Cell Type:-
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: DMEM:F12 Medium supplemented with:
- 0.005 mg/ml Insulin
- 0.01 mg/ml Transferrin
- 30nM Sodium selenite (final conc.)
- 10 nM Hydrocortisone (final conc.)
- 10 nM beta-estradiol (final conc.)
- extra 2mM L-glutamine (for final conc. of 4.5 mM)
- 5% fetal bovine serum (final conc.)
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Dilution, detach attached cells using 0.25% trypsin-0.03% EDTA
- Cell dissociation solution:
- Harvesting time:-
- Seeding density:-
- Recommended split ratio: 1:3 to 1:5
- Medium Renewal:-
- Subculturing method: Dilution, detach attached cells using 0.25% trypsin-0.03% EDTA
- Cryopreservation:
- Freezing medium:
- Complete growth medium + 5% DMSO
- Complete growth medium + 5% DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial: 1 ml
- Freezing medium:
- Applications:
- Cancer research
- 3D cell culture
- Availability:
- American Type Culture Collection (ATCC): Product link
- American Type Culture Collection (ATCC): Product link
- References
- Images:-
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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- Please let us know if you find any mistakes. Your comments are welcome in the comment box.
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