- Cell Line: NTERA-2
- Synonyms: NT-2
- Culture Type: Cell line
- Keywords: Human Embryonal Carcinoma (Teratocarcinoma) Cell Line
- Description: A subclone of TERA-2 cell line which was derived from a metastatic teratocarcinoma of a 22-year-old Caucasian male
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age: Adult, 22 year
- Ethnicity: Caucasian
- Gender: Male
- Species: Human (Homo sapiens)
- Tissue (origin):-
- Disease: Teratocarcinoma
- Year of origin:-
- Organism:
- Cell line features:
- Cell Type: Embryonal carcinoma
- Morphology: Epitheloid cells carrying strong granula
- Growth mode: Adherent, monolayer
- Life span: Infinite (continuous culture)
- Karyotype:-
- Doubling time: ~ 50-60 hours
- Special features:-
- Product:-
- Soft agar colony formation assay:-
- Tumorigenicity:-
- Gene expression:
- Sequence variations:
- Cell Type: Embryonal carcinoma
- Handling and maintenance:
- Biosafety level: BSL 1 (DSMZ) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: 85% DMEM + 10% h.i. FBS + 5% horse serum
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Enzymatic dissociation
- Cell dissociation solution: Trypsin/EDTA solution
- Harvesting time: Confluent culture
- Seeding density: 1 x 106 cells/80 cm2
- Recommended split ratio: 1:4
- Medium Renewal:-
- Subculturing method: Enzymatic dissociation
- Cryopreservation:
- Freezing medium: Complete growth medium + 20% (v/v) FBS + 10% (v/v) DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density): 2 x 106 cells/ml
- Volume per vial: 1 ml
- Freezing medium: Complete growth medium + 20% (v/v) FBS + 10% (v/v) DMSO
- Applications:
- Availability: DSMZ-German Collection of Microorganisms and Cell Cultures GmbH: Product link
- References
- Images:
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMEM (Dulbecco’s Modified Eagle Medium)
- DMSO (Dimethyl sulfoxide)
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