- Cell Line: Cell Line: Neuro-2a
- Synonyms: Neuro-2A, NEURO-2A
- Culture Type: Cell line
- Keywords: Mouse Neuroblastoma Cell Line
- Description: Established from the spontaneous tumor of a strain A albino mouse
- Origin:
- Organism:
- Species: Mouse (Mus musculus)
- Age:-
- Breed: strain A albino
- Gender:-
- Species: Mouse (Mus musculus)
- Tissue (origin): Brain
- Disease: Neuroblastoma
- Year of origin:-
- Organism:
- Cell line features:
- Cell Type: Neuroblast
- Morphology: Neuronal-like
- Growth mode: Adherent
- Life span: Infinite (continuous culture)
- Karyotype: Modal number = 95, range = 59 to 193
- Doubling time: ~70 hours
- Special features: produce large quantities of microtubular protein
- Product:-
- Soft agar colony formation assay:-
- Tumorigenicity:-
- Gene expression:
- Sequence variations:
- Cell Type: Neuroblast
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: Eagle’s Minimum Essential Medium + 10% (v/v) FBS
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Enzymatic dissociation
- Cell dissociation solution: Trypsin/EDTA solution
- Harvesting time: Subconfluent culture
- Seeding density: 2 – 4 x 104 cells/cm2
- Recommended split ratio: 1:3 to 1:6
- Medium Renewal: 1 to 2 times per week
- Subculturing method: Enzymatic dissociation
- Cryopreservation:
- Freezing medium: Complete growth medium + 5% (v/v) DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial: 1 ml
- Freezing medium: Complete growth medium + 5% (v/v) DMSO
- Applications:
- Availability: American Type Culture Collection (ATCC): Product link
- References
- Images: Neuro-2a cell line image link (Source: ATCC)
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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