Cell Line: SK-BR-3

  • Cell Line: SK-BR-3
  • Synonyms:
    • SK-BR-3
    • Sk-BR-3
    • Sk-Br-3
    • SK-BR3
    • SKBR-3
    • SKBr-3
    • SkBr-3
    • SK BR 03
    • SKBR3
  • Culture Type: Cell line
  • Keywords:  Human Breast Adenocarcinoma Cell Line
  • Description: Established from pleural effusion tumor cells of a 43-year-old Caucasian female patient with breast adenocarcinoma. The patient had been treated with radiation, steroids, cytoxan, and 5-fluorouracil.
  • Origin:
    • Organism:
      • Species: Human (Homo sapiens)
      • Ethnicity: Caucasian
      • Age: 43 years
      • Gender: Female
    • Tissue: Breast, Mammary gland
    • Disease: Breast adenocarcinoma
    • Primary/Metastasis: Metastatic
    • Collection site: Pleural effusion
    • Year of origin: 1970
    • Country of Origin: United States
  • Cell line features:
    • Cell Type: Epithelium?
    • Morphology: Epithelial-like
    • Growth mode: Adherent, monolayer
    • Life span: Infinite (continuous culture)
    • Doubling time:
    • Karyotype: Hypertriploid cell line, Modal chromosome number: 84
    • Microsatellite instability: Stable (MSS)
    • Special features:  Overexpresses the HER2/c-erb-2 gene product
    • Product:-
    • Soft agar colony formation assay:-
    • Tumorigenicity: Yes, in nude mice
    • Gene expression:
    • Sequence variations:
      • TP53 (R175H mutation, Oncogenic, Loss-of-function, Homozygous)
      • For more information, please visit:
        • https://depmap.org/portal/cell_line/SKBR3_BREAST?tab=mutations
        • https://depmap.org/portal/cell_line/SKBR3_BREAST?tab=fusions
  • Handling and maintenance:
    • Biosafety level: BSL 1 (It may differ in your country, follow the guidelines of your institute/country)
    • Complete culture medium: McCoy’s 5a Medium + 10% FBS (ATCC)
    • Culture conditions: 
      • Temperature: 37°C incubator
      • Atmosphere: 95% Air, 5% CO2
    • Subculturing:
      • Subculturing method: Enzymatic dissociation
      • Cell dissociation solution: 0.25% trypsin/0.53 mM EDTA solution
      • Harvesting time:-
      • Seeding density:-
      • Split ratio: 1:2
      • Split frequency:-
      • Medium Renewal: 2 to 3 times per week
    • Cryopreservation:
      • Freezing medium: Complete growth medium + 5% (v/v) DMSO
      • Storage conditions: Vapor phase of liquid nitrogen
      • Cell suspension (cell density):-
      • Volume per vial: 1 ml
  • Applications: 
    • Cancer research
    • 3D cell culture

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