Cell Line: SVCT-MI2

  • Cell Line: SVCT-MI2
  • Synonyms:
    • SVCT-MI2
  • Culture Type: Cell line
  • Keywords:  Human Breast Epithelial Transformed Cell Line
  • Description: Established by transforming breast epithelial cells with SV40
  • Origin:
    • Organism:
      • Species: Human (Homo sapiens)
      • Ethnicity:-
      • Age:-
      • Gender: Female
    • Tissue: Breast
    • Disease:-
    • Primary/Metastasis:-
    • Collection site:  Breast
    • Year of origin: 1987
    • Country of Origin:-
  • Cell line features:
    • Cell Type:-
    • Morphology:-
    • Growth mode: Adherent
    • Life span: Infinite (continuous culture)
    • Doubling time:-
    • Karyotype:-
    • Microsatellite instability:-
    • Special features:-
    • Product:-
    • Soft agar colony formation assay: Yes, form colonies
    • Tumorigenicity: Tumorigenic in nude mice
    • Gene expression:-
    • Sequence variations:
  • Handling and maintenance:
    • Biosafety level: BSL 2 (It may differ in your country, follow the guidelines of your institute/country)
    • Complete culture medium: RPMI 1640 or DMEM + 2mM Glutamine + 10 µg / ml insulin + 5 µg/ml hydrocortisone + 10% FBS
    • Culture conditions: 
      • Temperature: 37°C incubator
      • Atmosphere: 95% Air, 5% CO2
    • Subculturing:
      • Subculturing method: Enzymatic dissociation
      • Cell dissociation solution: Trypsin- EDTA solution
      • Harvesting time: sub-confluent cultures (70-80%)
      • Seeding density:-
      • Split ratio: 1:4 to 1:10
      • Split frequency:-
      • Medium Renewal:-
    • Cryopreservation:
      • Freezing medium: Complete growth medium + 20% (v/v) FBS + 10% (v/v) DMSO??
      • Storage conditions: Vapor phase of liquid nitrogen
      • Cell suspension (cell density): 1×106 cells/ml
      • Volume per vial: 1 ml
  • Applications: 
    • Cancer research
    • Drug Development

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