Cell Line: SK-LU-1

  • Cell Line: SK-LU-1
  • Synonyms:
    • SK-LU-1
    • SK-LU1
    • SK LU 1
    • SKLU1
    • SK-Lu1
    • SK-Lu-1
  • Culture Type: Cell line
  • Keywords:  Human Lung Adenocarcinoma Cell Line
  • Description: Established from the lungs of a 60-year-old, Caucasian female patient with adenocarcinoma
  • Origin:
    • Organism:
      • Species: Human (Homo sapiens)
      • Age:
      • Ethnicity: Caucasian
      • Gender: Female
    • Tissue: Lung
    • Disease: Adenocarcinoma
    • Primary/Metastasis: Primary (DepMap)
    • Collection site: Lung (DepMap)
    • Year of origin:-
  • Cell line features:
    • Cell Type: Epithelial
    • Morphology: Epithelial-like
    • Growth mode: Adherent, monolayer
    • Life span: Infinite (continuous culture)
    • Karyotype: Hypotetraploid
    • Doubling time:-
    • Microsatellite instability: stable
    • Special features:-
    • Product:
    • Soft agar colony formation assay:-
    • Tumorigenicity: tumorigenic in immunotolerant rats
    • Gene expression:
    • Sequence variations:
      • TP53 (H193R mutation, Likely Oncogenic, Likely Loss-of-function, Homozygous)
      • KRAS (G12D mutation, Oncogenic, Gain-of-function, Heterozygous)
      • For information, please visit
        • https://depmap.org/portal/cell_line/ACH-000309?tab=mutations
        • https://depmap.org/portal/cell_line/ACH-000309?tab=fusions
  • Handling and maintenance:
    • Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
    • Complete culture medium: Eagle’s Minimum Essential Medium + 10% FBS
    • Culture conditions: 
      • Temperature: 37°C incubator
      • Atmosphere: 95% Air, 5% CO2
    • Subculturing:
      • Subculturing method: Enzymatic dissociation
      • Cell dissociation solution: 0.25% (w/v) Trypsin- 0.53 mM EDTA solution
      • Harvesting time:-
      • Seeding density:-
      • Recommended split ratio: 1:2
      • Medium Renewal: Twice per week
    • Cryopreservation:
      • Freezing medium: Culture medium + 5% (v/v) DMSO
      • Storage conditions: Vapor phase of liquid nitrogen
      • Cell suspension (cell density):-
      • Volume per vial: 1 ml
  • Applications: 
    • 3D cell culture
    • Cancer research

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