Posted in Cancer/Tumor, Cell Line Database, Human Cell Line

Cell Line: SK-MES-1

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  • Cell Line: SK-MES-1
  • Synonyms:
    • SK-MES-1
    • SKMES1
    • SK MES 1
    • SK-MES1
    • SK-Mes-1
  • Culture Type: Cell line
  • Keywords:  Human Lung Squamous Cell Carcinomaa Cell Line 
  • Description: Established from the lungs of a 65-year-old, Caucasian male patient with squamous cell carcinoma
  • Origin:
    • Organism:
      • Species: Human (Homo sapiens)
      • Age: 65 years
      • Ethnicity: Caucasian
      • Gender: Male
    • Tissue: Lung
    • Disease: Squamous Cell Carcinoma
    • Primary/Metastasis: Metastatic (DepMap)
    • Collection site: Pleural effusion (ATCC)
    • Year of origin:-
  • Cell line features:
    • Cell Type: Epithelial
    • Morphology: Epithelial-like
    • Growth mode: Adherent, monolayer
    • Life span: Infinite (continuous culture)
    • Karyotype:
      • Hypotriploid (ATCC)
      • Human flat-moded hyperdiploid karyotype with 44% polyploidy (DSMZ)
    • Doubling time: ~50 hours (DSMZ)
    • Microsatellite instability: stable
    • Special features:-
    • Product:
    • Soft agar colony formation assay:-
    • Tumorigenicity:-
    • Gene expression:
    • Sequence variations:
      • For information, please visit
        • https://depmap.org/portal/cell_line/ACH-000665?tab=mutations
        • https://depmap.org/portal/cell_line/ACH-000665?tab=fusions
  • Handling and maintenance:
    • Biosafety level: BSL 1 (ATCC, DSMZ) (It may differ in your country, follow the guidelines of your institute/country)
    • Complete culture medium:
      • Eagle’s Minimum Essential Medium + 10% FBS (ATCC)
      • DMEM + 10-20% heat-inactivated FBS (DSMZ)
    • Culture conditions: 
      • Temperature: 37°C incubator
      • Atmosphere: 95% Air, 5% CO2
    • Subculturing:
      • Subculturing method: Enzymatic dissociation
      • Cell dissociation solution: 0.25% (w/v) Trypsin- 0.03 mM EDTA solution
      • Harvesting time:-
      • Seeding density:-
      • Recommended split ratio: 1:3 to 1:6
      • Medium Renewal: 2 to 3 times per week
    • Cryopreservation:
      • Freezing medium:
        • Culture medium + 5% (v/v) DMSO (ATCC)
        • Culture medium + 20% (v/v) FBS, 10% (v/v) DMSO (DSMZ)
      • Storage conditions: Vapor phase of liquid nitrogen
      • Cell suspension (cell density):-
      • Volume per vial: 1 ml
  • Applications: 
    • 3D cell culture
    • Cancer research

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