- Cell Line: SK-MEL-24
- Synonyms:
- SK-MEL-24
- SKMEL-24
- SKMEL24
- SKMel24
- SK-Mel24
- SK Mel 24
- Sk-Mel-24
- Culture Type: Cell line
- Keywords: Human Melanoma Cell Line
- Description: Established from the skin of a 67-year-old, Caucasian male patient with melanoma
- Origin:
- Cell line features:
- Cell Type:-
- Morphology: Stellate
- Growth mode: Adherent, monolayer
- Life span: Infinite (continuous culture)
- Doubling time: –
- Karyotype:-
- Microsatellite instability: Stable
- Special features:-
- Product:-
- Soft agar colony formation assay:-
- Tumorigenicity: Yes, tumorigenic in nude mice, forms malignant melanoma
- Gene expression:-
- Sequence variations: BRAF (V600E mutation, Oncogenic, Gain-of-function, Heterozygous)
- For more information, please visit:
- https://depmap.org/portal/cell_line/ACH-000822?tab=mutations
- https://depmap.org/portal/cell_line/ACH-000822?tab=fusions
- Cell Type:-
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: Eagle’s Minimum Essential Medium+ 15% FBS (ATCC)
- Culture conditions:
- Temperature: 37°C incubator
- Atmosphere: 95% Air, 5% CO2
- Temperature: 37°C incubator
- Subculturing:
- Cryopreservation:
- Freezing medium: Complete growth medium + 5% (v/v) DMSO (ATCC)
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial: 1 ml
- Freezing medium: Complete growth medium + 5% (v/v) DMSO (ATCC)
- Applications:
- 3D cell culture
- cancer research
- Cell Line Availability: The cell line can be obtained from the following sources.
- American Type Culture Collection (ATCC): Product link
- AcceGen™: Product link
- References
- Images:-
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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- Please let us know if you find any mistakes. Your comments are welcome in the comment box.
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