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Cell line: SK-MEL-3

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  • Cell Line: SK-MEL-3
  • Synonyms:
    • SK-MEL-3
    • SKMEL-3
    • SKMEL3
    • SKMel3
    • SK-Mel3
    • SK Mel 3
    • Sk-Mel-3
  • Culture Type: Cell line
  • Keywords:  Human Melanoma Cell Line
  • Description: Established from the lymph node metastasis of a malignant melanoma from a 42-year-old Caucasian woman
  • Origin:
    • Organism:
      • Species: Human (Homo sapiens)
      • Ethnicity: Caucasian
      • Age: 42 years
      • Gender: Female
    • Tissue: Skin (ATCC)
    • Disease:
    • Primary/Metastasis: Metastatic (DepMap)
    • Collection site: Lymph node (DepMap)
    • Year of origin:-
    • Country of Origin:-
  • Cell line features:
    • Cell Type: Melanoma
    • Morphology: Fibroblast-like
    • Growth mode: Adherent, monolayer
    • Life span: Infinite (continuous culture)
    • Doubling time: ~ 40-50 hours (DSMZ)
    • Karyotype: Human near-tetraploid karyotype with 3% polyploidy
    • Microsatellite instability: Stable
    • Special features:-
    • Product:-
    • Soft agar colony formation assay:-
    • Tumorigenicity: Yes, in nude mice; forms pigmented malignant melanoma (ATCC)
    • Gene expression:-
    • Sequence variations:
      • TP53 (R267W mutation, Likely Oncogenic, Likely Gain-of-function, Homozygous)
      • BRAF (V600E (c.1799T>A) mutation, Oncogenic, Gain-of-function, Zygosity=Heterozygous)
      • For more information, please visit:
        • https://depmap.org/portal/cell_line/ACH-000423?tab=mutations
        • https://depmap.org/portal/cell_line/ACH-000423?tab=fusions
  • Handling and maintenance:
    • Biosafety level: BSL 1 (ATCC, DSMZ) (It may differ in your country, follow the guidelines of your institute/country)
    • Complete culture medium: McCoy’s 5a Medium + 15% FBS  (ATCC)
    • Culture conditions: 
      • Temperature: 37°C incubator
      • Atmosphere: 95% Air, 5% CO2
    • Subculturing:
      • Subculturing method: Enzymatic dissociation
      • Cell dissociation solution: 0.25% (w/v) Trypsin- 0.53 mM EDTA solution (ATCC)
      • Harvesting time: confuent culture (DSMZ)
      • Seeding density:-
      • Split ratio: 1:3 to 1:5 (ATCC)
      • Split frequency:-
      • Medium Renewal: 2 to 3 times per week (ATCC)
    • Cryopreservation:
      • Freezing medium:
        • Complete growth medium + 5% (v/v) DMSO (ATCC)
        • Complete growth medium + 20% (v/v) FBS + 10% (v/v) DMSO (DSMZ)
      • Storage conditions: Vapor phase of liquid nitrogen
      • Cell suspension (cell density):-
      • Volume per vial: 1 ml
  • Applications: 
    • 3D cell culture
    • Cancer research
    • Toxicology
    • High-throughput screening
  • Cell Line Availability: The cell line can be obtained from the following sources.
    • American Type Culture Collection (ATCC): Product link
    • DSMZ-German Collection of Microorganisms and Cell Cultures GmbH: Product link

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