Cell Line: SK-MEL-28

  • Cell Line: SK-MEL-28
  • Synonyms:
    • SK-MEL-28
    • SKMEL-28
    • SKMEL28
    • SKMel28
    • SK-Mel28
    • SK Mel 28
    • Sk-Mel-28
  • Culture Type: Cell line
  • Keywords:  Human Melanoma Cell Line
  • Description: Established from the skin of a 51-year-old, male patient with malignant melanoma
  • Origin:
    • Organism:
      • Species: Human (Homo sapiens)
      • Ethnicity: Caucasian
      • Age: 51 years
      • Gender: Male
    • Tissue: Skin
    • Disease:
    • Primary/Metastasis: Primary (DepMap)
    • Collection site: Skin (DepMap)
    • Year of origin:-
    • Country of Origin:-
  • Cell line features:
    • Cell Type: Melanocyte
    • Morphology: Polygonal cells
    • Growth mode: Adherent, monolayer
    • Life span: Infinite (continuous culture)
    • Doubling time:
    • Karyotype: Modal number = 90, range = 81 to 96, hypotetraploid human cell line
    • Microsatellite instability: Stable
    • Special features: Lightly pigmented (Cellosaurus)
    • Product:-
    • Soft agar colony formation assay:-
    • Tumorigenicity: Tumorigenic in nude mice
    • Gene expression:-
    • Sequence variations:
      • PTEN (T167A mutation, Oncogenic, Loss-of-function, Homozygous)
      • CDK4 (R24C mutation, Oncogenic, Gain-of-function, Heterozygous)
      • TP53 (L145R mutation, Oncogenic, Loss-of-function, Homozygous)
      • BRAF (V600E mutation, Oncogenic, Gain-of-function, Homozygous)
      • For more information, please visit:
        • https://depmap.org/portal/cell_line/ACH-000615?tab=mutations
        • https://depmap.org/portal/cell_line/ACH-000615?tab=fusions
  • Handling and maintenance:
    • Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
    • Complete culture medium: Eagle’s Minimum Essential Medium+  10% FBS (ATCC)
    • Culture conditions: 
      • Temperature: 37°C incubator
      • Atmosphere: 95% Air, 5% CO2
    • Subculturing:
      • Subculturing method: Enzymatic dissociation
      • Cell dissociation solution: 0.25% (w/v) Trypsin- 0.53 mM EDTA solution (ATCC)
      • Harvesting time:
      • Seeding density:-
      • Split ratio: 1:3 to 1:8 (ATCC)
      • Split frequency:-
      • Medium Renewal: 2 to 3 times per week (ATCC)
    • Cryopreservation:
      • Freezing medium: Complete growth medium + 5% (v/v) DMSO (ATCC)
      • Storage conditions: Vapor phase of liquid nitrogen
      • Cell suspension (cell density):-
      • Volume per vial: 1 ml
  • Applications: 
    • 3D cell culture
    • Cancer research
    • Toxicology
    • High-throughput screening

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