Cell Line: SU-DHL-10

  • Cell Line: SU-DHL-10
  • Synonyms:
    • SU-DHL-10
    • SUDHL10
    • SUDHL-10
    • SuDHL-10
    • SuDHL 10
    • Stanford University-Diffuse Histiocytic Lymphoma-10
  • Culture Type: Cell line
  • Keywords:  Human B Cell Lymphoma Cell Line
  • Description: Established from the lymph node of a 25- year-old Caucasian male patient with Large Cell Lymphoma
  • Origin:
    • Organism:
      • Species: Human (Homo sapiens)
      • Age: 25 years
      • Ethnicity: Caucasian
      • Gender: Male
    • Tissue: Lymph node
    • Disease: Large Cell Lymphoma
    • Primary/Metastasis: Metastatic (DepMap)
    • Collection site:
      • Peritoneal effusion (ATCC)
      • Pleural effusion  (DepMap)
    • Year of origin: 1978
    • Country of Origin:-
  • Cell line features:
    • Cell Type: B lymphocyte
    • Morphology: Lymphoblast-like, round lymphoid cells growing singly or in clumps
    • Growth mode: Suspension
    • Life span: Infinite (continuous culture)
    • Karyotype: hyperdiploid karyotype (DSMZ)
    • Doubling time: ~ 15-30 hours (DSMZ)
    • Microsatellite instability: Stable
    • Special features:-
    • Product:-
    • Soft agar colony formation assay:-
    • Tumorigenicity:-
    • Gene expression:-
    • Sequence variations:
      • PTEN (Y68H mutation, Likely Oncogenic, Likely Loss-of-function, Homozygous)
      • PTEN (D162H mutation, Oncogenic, Loss-of-function)
      • EP300 (L415P mutation, Likely Oncogenic, Unknown)
      • CCND3 (I290T mutation, Likely Oncogenic, Likely Gain-of-function)
      • EZH2 (Y646F mutation, Likely Oncogenic, Likely Gain-of-function)
      • For information, please visit
        • https://depmap.org/portal/cell_line/ACH-000271?tab=mutations
        • https://depmap.org/portal/cell_line/ACH-000271?tab=fusions
  • Handling and maintenance:
    • Biosafety level: BSL 1 (ATCC, DSMZ) (It may differ in your country, follow the guidelines of your institute/country)
    • Complete culture medium:
      • RPMI-1640 Medium + 10% FBS
    • Culture conditions: 
      • Temperature: 37°C incubator
      • Atmosphere: 95% Air, 5% CO2
    • Subculturing:
      • Subculturing method: Dilution
      • Cell dissociation solution: NA
      • Harvesting time: 1.0 x 106 and 2.0 x 106 cells/mL. (ATCC)
      • Seeding density: 8.0 x 104 to 2.0 x 105 cells/mL (ATCC)
      • Recommended split ratio: 1:4 to 1:12
      • Medium Renewal: every 2 to 4 days
    • Cryopreservation:
      • Freezing medium: Complete growth medium + 20% (v/v) FBS + 10% (v/v) DMSO (ATCC)
      • Storage conditions: Vapor phase of liquid nitrogen
      • Cell suspension (cell density):-
      • Volume per vial: 1 ml
  • Applications: 
    • 3D cell culture
    • Immunology
  • Cell Line Availability: The cell line can be obtained from the following sources.
    • American Type Culture Collection (ATCC): Product link
    • DSMZ-German Collection of Microorganisms and Cell Cultures GmbH: Product link

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