- Cell Line: SU-DHL-16
- Synonyms:
- SU-DHL-16
- SUDHL16
- SUDHL-16
- SuDHL-16
- SuDHL 16
- Stanford University-Diffuse Histiocytic Lymphoma-16
- Culture Type: Cell line
- Keywords: Human B Cell Lymphoma Cell Line
- Description: Established from the lymph node of a Caucasian male patient with Large Cell Lymphoma
- Origin:
- Cell line features:
- Cell Type: B lymphocyte
- Morphology: Lymphoblast-like, round to polymorph cells growing in clumps
- Growth mode: Suspension
- Life span: Infinite (continuous culture)
- Karyotype: Human flat-moded hyperdiploid karyotype with 3% polyploidy (DSMZ)
- Doubling time: ~ 40 hours (DSMZ)
- Microsatellite instability: Instable
- Special features:-
- Product:-
- Soft agar colony formation assay:-
- Tumorigenicity:-
- Gene expression: Methionine aminopeptidase 2 (MetAP2), Cathelicidin antimicrobial peptide (CAMP)
- Sequence variations:
- CREBBP (S1680del mutation, Likely Oncogenic, Likely Loss-of-function)
- CREBBP (Y1450C mutation, Likely Oncogenic, Likely Loss-of-function)
- XPO1 (E571A mutation, Likely Oncogenic, Likely Gain-of-function)
- CARD11 (D357V m mutation, Likely Oncogenic, Likely Gain-of-function)
- For information, please visit
- https://depmap.org/portal/cell_line/ACH-002307?tab=mutations
- https://depmap.org/portal/cell_line/ACH-002307?tab=fusions
- CREBBP (S1680del mutation, Likely Oncogenic, Likely Loss-of-function)
- Cell Type: B lymphocyte
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC, DSMZ) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium:
- RPMI-1640 Medium + 10% FBS
- RPMI-1640 Medium + 10% FBS
- Culture conditions:
- Temperature: 37°C incubator
- Atmosphere: 95% Air, 5% CO2
- Temperature: 37°C incubator
- Subculturing:
- Cryopreservation:
- Freezing medium: Complete growth medium + 20% (v/v) FBS + 10% (v/v) DMSO (ATCC)
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial: 1 ml
- Freezing medium: Complete growth medium + 20% (v/v) FBS + 10% (v/v) DMSO (ATCC)
- Applications:
- 3D cell culture
- Immunology
- Cell Line Availability: The cell line can be obtained from the following sources.
- American Type Culture Collection (ATCC): Product link
- DSMZ-German Collection of Microorganisms and Cell Cultures GmbH: Product link
- References
- Images:
- SU-DHL-16 cell line image link 1 (Source: ATCC)
- SU-DHL-16 cell line image link 2 (Source: DSMZ)
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
- Article Authenticity Index *
- Please let us know if you find any mistakes. Your comments are welcome in the comment box.
Was this post helpful?
Let us know if you liked the post. That’s the only way we can improve.
