Cell Line: SUP-T1

  • Cell Line: SUP-T1
  • Synonyms:
    • SUP-T1
    • SUPT1
    • SUPT-1
    • SUP T-1
    • Sup-T1
    • Sup T-1
    • SupT1
    • Stanford University Pediatric T-cell line 1
  • Culture Type: Cell line
  • Keywords:  Human T Cell Lymphoma Cell Line
  • Description: Established from pleural effusion of an 8 year old Caucasian child with T-Cell Lymphoblastic Lymphoma
  • Origin:
    • Organism:
      • Species: Human (Homo sapiens)
      • Age: 8 years
      • Ethnicity: Caucasian
      • Gender: Male
    • Tissue: Blood/Lymphoid
    • Disease: T-Cell Lymphoblastic Lymphoma
    • Primary/Metastasis: Metastatic (DepMap)
    • Collection site: Pleural effusion
    • Year of origin:
    • Country of Origin: United States
  • Cell line features:
    • Cell Type: T lymphoblast
    • Morphology: Lymphoblast-like; single, round cells
    • Growth mode: Suspension
    • Life span: Infinite (continuous culture)
    • Doubling time: ~ 30 hours (DSMZ)
    • Karyotype: Human hypotetraploid karyotype with 1.8% polyploidy (DSMZ
    • Microsatellite instability:
    • Special features: The cells express multiple T lineage markers
    • Product:-
    • Soft agar colony formation assay:-
    • Tumorigenicity:-
    • Gene expression:-
    • Sequence variations:
      • MPL (A497T mutation, Likely Oncogenic, Likely Gain-of-function)
      • INPPL1 (R70H mutation, Likely Oncogenic, Unknown)
      • EP300 (L415P mutation, Likely Oncogenic, Unknown)
      • TP53 (R267L mutation, Likely Oncogenic, Likely Gain-of-function, Heterozygous)
      • TP53 (R273H mutation, Oncogenic, Loss-of-function)
      • TP53 (R248Q mutation, Likely Oncogenic, Likely Loss-of-function)
      • PIK3CA (E970K mutation, Likely Oncogenic, Likely Gain-of-function)
      • CARD11 (E134K mutation, Likely Oncogenic, Likely Gain-of-function)
      • PIK3CA (E545D mutation, Likely Oncogenic, Likely Gain-of-function, Heterozygous)
      • For information, please visit
        • https://depmap.org/portal/cell_line/ACH-000953?tab=mutations
        • https://depmap.org/portal/cell_line/ACH-000953?tab=fusions
  • Handling and maintenance:
    • Biosafety level: BSL 1 (ATCC, DSMZ) (It may differ in your country, follow the guidelines of your institute/country)
    • Complete culture medium: RPMI-1640 Medium + 10% FBS
    • Culture conditions: 
      • Temperature: 37°C incubator
      • Atmosphere: 95% Air, 5% CO2
    • Subculturing:
      • Subculturing method: Dilution
      • Cell dissociation solution: NA
      • Harvesting time: 2 x 106 cells/mL
      • Seeding density: 3 x 105 viable cells/mL
      • Recommended split ratio: 1:4 to 1:6
      • Medium Renewal: 2 to 3 times per week
    • Cryopreservation:
      • Freezing medium: Complete growth medium + 5% (v/v) DMSO (ATCC)
      • Storage conditions: Vapor phase of liquid nitrogen
      • Cell suspension (cell density):-
      • Volume per vial: 1 ml
  • Applications: 
    • 3D cell culture
    • Immunology
    • Transfection host
    • Useful for studies of cell fusion and cytopathic effects of HIV-1 as well as cytopathic isolates of HIV-2
    • Susceptible to infection by herpesvirus 6B (HHV-6B) strain Z29
  • Cell Line Availability: The cell line can be obtained from the following sources.

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