Cell Line: SUP-B15

  • Cell Line: SUP-B15
  • Synonyms:
    • SUP-B15
    • SUPB-15
    • SUPB15
    • Sup-B15
  • Culture Type: Cell line
  • Keywords:  Human B Lymphoblast Cell Line
  • Description: Established from the bone marrow of a White, 8-year-old, male patient with acute lymphoblastic leukemia (ALL). (ATCC
  • Origin:
    • Organism:
    • Tissue: Bone Marrow/Lymphoid
    • Disease: Acute lymphoblastic leukemia (ALL)
    • Primary/Metastasis: Primary (DepMap)
    • Collection site: Bone marrow
    • Year of origin: 1984 (DSMZ)
    • Country of Origin:-
  • Cell line features:
    • Cell Type: B lymphoblast
    • Morphology: Lymphoblast-like; single, round cells
    • Growth mode: Suspension
    • Life span: Infinite (continuous culture)
    • Doubling time:
    • Karyotype: Human pseudodiploid karyotype
    • Microsatellite instability: Stable (Cellosaurus)
    • Special features: The cells express multiple B lineage markers, but do not express T cell markers.
    • Product:-
    • Soft agar colony formation assay:-
    • Tumorigenicity:-
    • Gene expression:-
    • Sequence variations:
      • For information, please visit
        • https://depmap.org/portal/cell_line/ACH-000059?tab=mutations
        • https://depmap.org/portal/cell_line/ACH-000059?tab=fusions
  • Handling and maintenance:
    • Biosafety level: BSL 1 (ATCC, DSMZ) (It may differ in your country, follow the guidelines of your institute/country)
    • Complete culture medium: 
      • Iscove’s modified Dulbecco’s medium  (4 mM L-glutamine, 1.5 g/L sodium bicarbonate) + 20% FBS (ATCC)
      • McCoy’s 5A + 20% heat inactivated FBS (DSMZ)
    • Culture conditions: 
      • Temperature: 37°C incubator
      • Atmosphere: 95% Air, 5% CO2
    • Subculturing:
      • Subculturing method: Dilution
      • Cell dissociation solution: NA
      • Harvesting time: 2 x 106 cells/mL
      • Seeding density: 5 x 105 viable cells/mL
      • Recommended split ratio: 1:2 to 1:4
      • Medium Renewal: 2 to 3 times per week
    • Cryopreservation:
      • Freezing medium: Complete growth medium + 5% (v/v) DMSO (ATCC)
      • Storage conditions: Vapor phase of liquid nitrogen
      • Cell suspension (cell density):-
      • Volume per vial: 1 ml
  • Applications: 
    • 3D cell culture
    • Immunology
  • Cell Line Availability: The cell line can be obtained from the following sources.
    • American Type Culture Collection (ATCC): Product link
    • DSMZ-German Collection of Microorganisms and Cell Cultures GmbH: Product link  

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