- Cell Line: SUP-T11
- Synonyms:
- SUP-T11
- SupT11
- SUPT11
- Stanford University Pediatric T-cell line 11
- Culture Type: Cell line
- Keywords: Human T Cell Leukemia Cell Line
- Description: Established from the bone marrow of a 74-year-old man with T-ALL. Cell line was established in the presence of IGF-I under hypoxic conditions (DSMZ)
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age: 74 years
- Ethnicity: Caucasian
- Gender: Male
- Tissue: Bone marrow
- Disease: T-cell acute lymphoblastic leukemia
- Primary/Metastasis:-
- Collection site: Bone marrow
- Year of origin:-
- Country of Origin:-
- Organism:
- Cell line features:
- Cell Type: T-cell
- Morphology: Small, round to polymorph cells
- Growth mode: Suspension, single cells
- Life span: Infinite (continuous culture)
- Karyotype: Human highly rearranged hypodiploid karyotype with 5% polyploidy
- Doubling time: ~35-45 hours
- Microsatellite instability:-
- Special features: Express high levels of TCL1A and TCL1B genes due to their juxtaposition from chromosome 14q32.1 to the regulatory element of the T-cell receptor C alpha/delta locus at chromosome 14q11
- Product:-
- Soft agar colony formation assay:-
- Tumorigenicity:-
- Gene expression:-
- Sequence variations:
- For information, please visit
- https://depmap.org/portal/cell_line/ACH-000122?tab=mutations
- https://depmap.org/portal/cell_line/ACH-000122?tab=fusions
- For information, please visit
- Cell Type: T-cell
- Handling and maintenance:
- Biosafety level: BSL 1 (DSMZ) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium:
- RPMI-1640 Medium + 10% FBS
- RPMI-1640 Medium + 10% FBS
- Culture conditions:
- Temperature: 37°C incubator
- Atmosphere: 95% Air, 5% CO2
- Temperature: 37°C incubator
- Subculturing:
- Subculturing method: Dilution
- Cell dissociation solution: NA
- Harvesting time: 1.5 x 106 cells/ml
- Seeding density: 0.5 x 106 cells/ml
- Recommended split ratio: 1:2 to 1:4
- Medium Renewal:-
- Subculturing method: Dilution
- Cryopreservation:
- Freezing medium: Complete growth medium + 20% (v/v) FBS + 10% (v/v) DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial: 1 ml
- Freezing medium: Complete growth medium + 20% (v/v) FBS + 10% (v/v) DMSO
- Applications:
- Immunology
- Immunology
- Cell Line Availability: The cell line can be obtained from the following sources.
- DSMZ-German Collection of Microorganisms and Cell Cultures GmbH: Product link
- References
- Images:
- SUP-T11 cell line image link 1 (Source: DSMZ)
- SUP-T11 cell line image link 2 (Source: DSMZ)
- SUP-T11 cell line image link 3 (Source: DSMZ)
- SUP-T11 cell line image link 4 (Source: DepMap)
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
- Article Authenticity Index *
- Please let us know if you find any mistakes. Your comments are welcome in the comment box.
