Cell Line: SUP-T11

  • Cell Line: SUP-T11
  • Synonyms:
    • SUP-T11
    • SupT11
    • SUPT11
    • Stanford University Pediatric T-cell line 11
  • Culture Type: Cell line
  • Keywords:   Human T Cell Leukemia Cell Line
  • Description: Established from the bone marrow of a 74-year-old man with T-ALL. Cell line was established in the presence of IGF-I under hypoxic conditions (DSMZ)
  • Origin:
    • Organism:
      • Species: Human (Homo sapiens)
      • Age: 74 years
      • Ethnicity: Caucasian
      • Gender: Male
    • Tissue: Bone marrow
    • Disease: T-cell acute lymphoblastic leukemia
    • Primary/Metastasis:-
    • Collection site: Bone marrow
    • Year of origin:-
    • Country of Origin:-
  • Cell line features:
    • Cell Type: T-cell
    • Morphology: Small, round to polymorph cells
    • Growth mode: Suspension, single cells
    • Life span: Infinite (continuous culture)
    • Karyotype: Human highly rearranged hypodiploid karyotype with 5% polyploidy
    • Doubling time: ~35-45 hours
    • Microsatellite instability:-
    • Special features: Express high levels of TCL1A and TCL1B genes due to their juxtaposition from chromosome 14q32.1 to the regulatory element of the T-cell receptor C alpha/delta locus at chromosome 14q11
    • Product:-
    • Soft agar colony formation assay:-
    • Tumorigenicity:-
    • Gene expression:-
    • Sequence variations:
      • For information, please visit
        • https://depmap.org/portal/cell_line/ACH-000122?tab=mutations
        • https://depmap.org/portal/cell_line/ACH-000122?tab=fusions
  • Handling and maintenance:
    • Biosafety level: BSL 1 (DSMZ) (It may differ in your country, follow the guidelines of your institute/country)
    • Complete culture medium:
      • RPMI-1640 Medium + 10% FBS
    • Culture conditions: 
      • Temperature: 37°C incubator
      • Atmosphere: 95% Air, 5% CO2
    • Subculturing:
      • Subculturing method: Dilution
      • Cell dissociation solution: NA
      • Harvesting time: 1.5 x 106 cells/ml
      • Seeding density: 0.5 x 106 cells/ml
      • Recommended split ratio: 1:2 to 1:4
      • Medium Renewal:-
    • Cryopreservation:
      • Freezing medium: Complete growth medium + 20% (v/v) FBS + 10% (v/v) DMSO
      • Storage conditions: Vapor phase of liquid nitrogen
      • Cell suspension (cell density):-
      • Volume per vial: 1 ml
  • Applications: 
    • Immunology
  • Cell Line Availability: The cell line can be obtained from the following sources.
    • DSMZ-German Collection of Microorganisms and Cell Cultures GmbH: Product link

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