Cell Line: SVCT

  • Cell Line: SVCT
  • Synonyms:
    • SVCT
  • Culture Type: Cell line
  • Keywords:  Human Breast Epithelial Transformed Cell Line
  • Description: Established by transforming breast epithelial cells with SV40
  • Origin:
    • Organism:
      • Species: Human (Homo sapiens)
      • Ethnicity:-
      • Age:-
      • Gender: Female
    • Tissue: Breast
    • Disease:-
    • Primary/Metastasis:-
    • Collection site:  Breast
    • Year of origin:-
    • Country of Origin:-
  • Cell line features:
    • Cell Type: Epithelium
    • Morphology: Epithelial-like
    • Growth mode: Adherent, dome formation when confluent
    • Life span: Infinite (continuous culture)
    • Doubling time:-
    • Karyotype:-
    • Microsatellite instability:-
    • Special features:-
    • Product:-
    • Soft agar colony formation assay:-
    • Tumorigenicity: Do not form tumor in athymic male nude mice
    • Gene expression: positive for the expression of keratin 7, 8 and 18 but negative for keratin 19
    • Sequence variations:
  • Handling and maintenance:
    • Biosafety level: BSL 2 (It may differ in your country, follow the guidelines of your institute/country)
    • Complete culture medium: DMEM + 2mM Glutamine + 5µg/ml Hydrocortisone + 10µg/ml Insulin + 10% FBS (sigmaaldrich)
    • Culture conditions: 
      • Temperature: 37°C incubator
      • Atmosphere: 95% Air, 5% CO2
    • Subculturing:
      • Subculturing method: Enzymatic dissociation
      • Cell dissociation solution: Trypsin- EDTA solution
      • Harvesting time: sub-confluent cultures (70-80%)
      • Seeding density:-
      • Split ratio: 1:2 to 1:4
      • Split frequency:-
      • Medium Renewal:-
    • Cryopreservation:
      • Freezing medium: Complete growth medium + 20% (v/v) FBS + 10% (v/v) DMSO??
      • Storage conditions: Vapor phase of liquid nitrogen
      • Cell suspension (cell density): 1×106 cells/ml
      • Volume per vial: 1 ml
  • Applications: 
    • Cancer research
    • Drug Development

Was this post helpful?

Author: admin

Leave a Reply

Your email address will not be published. Required fields are marked *