Cell Line: SW-626

• Cell Line: SW-626
  
• Synonyms:
  
  • SW-626
  • SW 626
    
  • SW626
• Culture Type: Cell line
  
• Keywords:  Human Adenocarcinoma Cell Line
  
• Description: Established from the ovaries of a 46-year-old, Caucasian female patient with adenocarcinoma. A recent report suggest that the cell line might have been derived from an ovarian metastasis of a primary adenocarcinoma of the colon
  
• Origin:
  • Organism:
    • Species: Human (Homo sapiens)
    • Age: 46 years
      
    • Ethnicity: Caucasian
      
    • Gender: Female
      
  • Tissue: Ovary ^#
    
  • Disease: Adenocarcinoma
    
  • Primary/Metastasis: Metastatic (DepMap)
    
  • Collection site: Ovary (DepMap)
    
  • Year of origin: 1974
    
  • Country of Origin: United States
    
• Cell line features:
  • Cell Type: Epithelial
    
  • Morphology: Epithelial-like
    
  • Growth mode: Adherent
    
  • Life span: Infinite (continuous culture)
  • Karyotype: Hypertetraploid, modal number = 104
    
  • Doubling time:-
    
  • Microsatellite instability: Stable
    
  • Special features:-
    
  • Product:-
    
  • Soft agar colony formation assay:-
  • Tumorigenicity: tumorigenic in nude mice 
    
  • Gene expression:-
    
  • Sequence variations:
    
    • KRAS (G12V mutation, Oncogenic, Gain-of-function, Heterozygous)
    • TP53 (G262V mutation, Likely Oncogenic, Likely Loss-of-function,  Homozygous)
      
    • For information, please visit
      
      • https://depmap.org/portal/cell_line/ACH-001399?tab=mutations
      • https://depmap.org/portal/cell_line/ACH-001399?tab=fusions
        
• Handling and maintenance:
  • Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
  • Complete culture medium:
    • Leibovitz’s L-15 Medium + 10% FBS (ATCC)
      
  • Culture conditions: 
    • Temperature: 37°C incubator
      
    • Atmosphere: 100% Air
      
  • Subculturing:
    • Subculturing method: Enzymatic dissociation
      
    • Cell dissociation solution: 0.25% (w/v) Trypsin- 0.53 mM EDTA solution
      
    • Harvesting time: Subconfluent culture (70% confluency) (Cells do not reach confluency but will grow in small islands)
      
    • Seeding density:-
      
    • Recommended split ratio: 1:2 to 1:5
      
    • Medium Renewal: 2 to 3 times per week
      
  • Cryopreservation:
    • Freezing medium: Complete growth medium + 5% (v/v) DMSO
      
    • Storage conditions: Vapor phase of liquid nitrogen
    • Cell suspension (cell density):-
      
    • Volume per vial: 1 ml
      
• Applications: 
  • 3D cell culture
  • Cancer research
    

• Availability:
  • American Type Culture Collection (ATCC): Product link
  • UK Health Security Agency Culture Collections: Product link
  • AcceGen: Product link

• References
  • ATCC
  • Culturecollections
  • Cellosaurus
    
  • DepMap
• Images:
  • SW-626 cell line image link 1 (Source: DepMap)
• Abbreviations
  • BSL (Biosafety level)
  • FBS (Fetal Bovine Serum)
  • DMSO (Dimethyl sulfoxide)
• Article Authenticity Index *
  • Please let us know if you find any mistakes. Your comments are welcome in the comment box.

^# A recent report suggest that the cell line might have been derived from an ovarian metastasis of a primary adenocarcinoma of the colon