- Cell Line: SW-1463
- Synonyms:
- SW-1463
- SW 1463
- SW1463
- Culture Type: Cell line
- Keywords: Human Colorectal Adenocarcinoma Cell Line
- Description: Established from a 66-year-old Caucasian female patient with Colorectal adenocarcinoma (Dukes’ type C)
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age: 66 years
- Ethnicity: Caucasian
- Gender: female
- Tissue: Large intestine, Rectum
- Disease: Colorectal adenocarcinoma (Dukes’ type C)
- Primary/Metastasis: Large intestine (DepMap)
- Collection site: Rectum
- Year of origin:-
- Country of Origin: United States
- Organism:
- Cell line features:
- Cell Type: Epithelial
- Morphology: Epithelial-like
- Growth mode: Adherent
- Life span: Infinite (continuous culture)
- Karyotype: Hypertriploid
- Doubling time:-
- Microsatellite instability: Stable
- Special features: positive for keratin by immunoperoxidase staining
- Product: Carcinoembryonic antigen (CEA)
- Soft agar colony formation assay:-
- Tumorigenicity: tumorigenic in nude mice
- Gene expression:-
- Sequence variations:
- KRAS (G12C mutation, Oncogenic, Gain-of-function, Homozygous)
- TP53 (R248Q mutation, Likely Oncogenic, Likely Loss-of-function, Homozygous)
- For information, please visit
- https://depmap.org/portal/cell_line/ACH-000470?tab=mutations
- https://depmap.org/portal/cell_line/ACH-000470?tab=fusions
- Cell Type: Epithelial
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium:
- Leibovitz’s L-15 Medium + 10% FBS
- Leibovitz’s L-15 Medium + 10% FBS
- Culture conditions:
- Temperature: 37°C incubator
- Atmosphere: 100% Air
- Temperature: 37°C incubator
- Subculturing:
- Subculturing method: Enzymatic dissociation
- Cell dissociation solution: 0.25% (w/v) Trypsin- 0.53 mM EDTA solution
- Harvesting time:-
- Seeding density:-
- Recommended split ratio: 1:3 to 1:8
- Medium Renewal: 1 to 2 times per week
- Subculturing method: Enzymatic dissociation
- Cryopreservation:
- Freezing medium:
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial: 1 ml
- Applications:
- 3D cell culture
- Cancer research
- Cell Line Availability: The cell line can be obtained from the following sources.
- American Type Culture Collection (ATCC): Product link
- UK Health Security Agency Culture Collections: Product link
- Cytion: Product link
- References
- Images:
- SW-1463 cell line image link 1 (Source: Culturecollections)
- SW-1463 cell line image link 2 (Source: Cytion)
- SW-1463 cell line image link 3 (Source: Cytion)
- SW-1463 cell line image link 4 (Source: Cytion)
- SW-1463 cell line image link 5 (Source: Cytion)
- SW-1463 cell line image link 6 (Source: DepMap)
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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