- Cell Line: SW-837
- Synonyms:
- SW-837
- SW 837
- SW837
- Culture Type: Cell line
- Keywords: Human Colorectal Adenocarcinoma Cell Line
- Description: Established from a 53-year-old Caucasian male patient with stage IV colorectal adenocarcinoma
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age: 53 years
- Ethnicity: Caucasian
- Gender: Male
- Tissue: Rectum
- Disease: Rectal adenocarcinoma, grade IV
- Primary/Metastasis: Primary
- Collection site: large intestine
- Year of origin:-
- Organism:
- Cell line features:
- Cell Type:-
- Morphology: Epithelial-like
- Growth mode: Adherent
- Life span: Infinite (continuous culture)
- Karyotype:
- Doubling time: 40.60 hours (Cellosaurus)
- Microsatellite instability: stable
- Special features: positive for keratin by immunoperoxidase staining
- Product:
- Soft agar colony formation assay:-
- Tumorigenicity: tumorigenic in nude mice
- Gene expression:
- Sequence variations:
- TP53 (R248W mutation, Likely Oncogenic, Loss-of-function, Hemizygous)
- KRAS (G12C mutation, Oncogenic, Gain-of-function, Heterozygous)
- For information, please visit
- https://depmap.org/portal/cell_line/ACH-000421?tab=mutations
- https://depmap.org/portal/cell_line/ACH-000421?tab=fusions
- Cell Type:-
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: Leibovitz’s L-15 Medium + 10% FBS
- Culture conditions:
- Temperature: 37°C incubator
- Atmosphere: 100% Air
- Temperature: 37°C incubator
- Subculturing:
- Subculturing method: Enzymatic dissociation
- Cell dissociation solution: 0.25% (w/v) Trypsin- 0.53 mM EDTA solution
- Harvesting time:-
- Seeding density:-
- Recommended split ratio: 1:2 to 1:5
- Medium Renewal: 1 to 2 times per week
- Subculturing method: Enzymatic dissociation
- Cryopreservation:
- Freezing medium: Culture medium + 50% FBS + 10% DMSO (Cabri-ICLC HTL99021)
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial: 1 ml
- Freezing medium: Culture medium + 50% FBS + 10% DMSO (Cabri-ICLC HTL99021)
- Applications:
- High-throughput screening
- 3D cell culture
- Toxicology
- transfection host
- Availability:
- American Type Culture Collection (ATCC): Product link
- UK Health Security Agency Culture Collections: Product link
- References
- Images:
- SW-837 cell line image link (Source: DepMap)
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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