- Cell Line: Hs 852.T
- Synonyms:
- Hs852.T
- HS852T
- Hs852T
- Hs-852-T
- HS852.T
- Culture Type: Cell line
- Keywords: Human epithelial melanoma cell line
- Description: Established from the skin of a 53-year-old, Asian male patient with melanoma
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age: 53 years
- Ethnicity: Asian
- Gender: Male
- Tissue: Skin
- Disease:
- Melanoma
- Melanoma
- Year of origin:-
- Organism:
- Cell line features:
- Cell Type:-
- Morphology: Epithelial
- Growth mode: Adherent
- Life span: Infinite (continuous culture)
- Karyotype: modal number = 84; range = 42 to 95
- Doubling time:-
- Gene expression:-
- Sequence variations:
- NRAS (G12V mutation, Oncogenic Gain-of-function)
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- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: DMEM + 10% FBS
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Enzymatic dissociation
- Cell dissociation solution: 0.25% (w/v) Trypsin-0.53 mM EDTA solution
- Harvesting time:-
- Split ratio:-
- Medium Renewal:-
- Cryopreservation:
- Freezing medium: Complete growth medium + 5% DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial:-
- Freezing medium: Complete growth medium + 5% DMSO
- Applications:
- Cancer research
- 3D cell culture
- Cancer research
- Availability:
- American Type Culture Collection (ATCC): Product link
- ITI BioChem: Product link
- References:
- Images:-
- Hs 852.T cell image (Source: ATCC)
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
- DMEM (Dulbecco’s Modified Eagle’s Medium)
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