Tag: plasmid

Chloramphenicol Amplification of Low-copy Number Plasmids

Low-copy number plasmid vectors such as those based on pMB1 or ColE1 can be amplified by adding chloramphenicol to the…

Plasmid: pBR322 (Cloning Vector)

Plasmid name pBR322 Application Cloning vector Replicon pMBI Host E. coli DH5α, and other related strains Plasmid size (kbp) 4.361…

Plasmid Isolation: Genomic DNA vs Plasmid DNA

The major task in plasmid isolation methods is to separate Plasmid DNA from genomic DNA. Here we have compared some…

Plasmid: pSymA

Plasmid name pSymA Host Sinorhizobium meliloti Plasmid size(kbp) 1354.2 Plasmid geometry Circular Plasmid copy number 2–3

Plasmid: SCP1

Plasmid name SCP1 Host Streptomyces coelicolor Plasmid size(kbp) 350.0 Plasmid geometry Linear Plasmid copy number 4

Plasmid: F

Plasmid name F Host Escherichia coli Plasmid size(kbp) 99.2 Plasmid geometry Circular Plasmid copy number 1–2

Plasmid: lp25

Plasmid name lp25 Host Borrelia burgdorferi Plasmid size(kbp) 24.2 Plasmid geometry Linear Plasmid copy number 1–2

Plasmid: ColE1

Plasmid name ColE1 Host Escherichia coli Plasmid size(kbp) 6.6 Plasmid geometry Circular Plasmid copy number 10–30

Plasmid: pUB110

Plasmid name pUB110 Host Bacillus subtilis Plasmid size(kbp) 2.3 Plasmid geometry Circular Plasmid copy number 20–50

Plasmid: Timeline of Key Events

1903: The chromosome theory of inheritance was developed independently by two researchers: Walter S. Sutton and Theodor Boveri 1910: The…

Plasmid vs Virus

Properties Plasmid Virus Remarks Replication Autonomous, requires host enzyme Autonomous, requires host enzyme   Relationship with host More symbiotic than…

Plasmid vs Episome

Properties Plasmid Episome Remarks Molecular nature DNA DNA Both of them are made up of deoxyribonucleic acid Genetic material type…

Linear Plasmid

The Borrelia burgdorferi genome consists of a linear chromosome and at least 21 plasmids of which 12 are linear and…

Plasmid Overview

Plasmid

The term “Plasmid” was first coined by Joshua Lederberg in 1952 (Lederberg, 1952). Plasmids are extrachromosomal, double-stranded, covalently closed circular…

List of Naturally Occurring Plasmids in  Escherichia coli

pEC156 ColE1 pCoo REFERENCES Werbowy & Kaczorowski, 2016. Plasmid pEC156, a Naturally Occurring Escherichia coli Genetic Element That Carries Genes…