Tag: plasmid
Plasmidome
The plasmidome represents the complete plasmid content within a microbial community or environment, encompassing the collective genetic information carried by…
pEC156
pEC156 is a naturally occurring plasmid found in Escherichia coli, classified as a small, high-copy-number, non-conjugative but mobilizable plasmid. With…
Factors Influencing Plasmid Host-Range Specificity in Bacteria
The host-range specificity of a plasmid refers to the spectrum of bacterial species in which the plasmid can successfully replicate…
Plasmid: Geometry and Conformation
Plasmid geometry and conformation represent fundamental aspects of plasmid structure that influence their biological function and behavior in both natural…
Plasmid DNA Isolation by the Alkaline Lysis Method Vs Boiling Lysis Method
Criteria Alkaline Lysis Method Boiling Lysis Method Remarks Principle Selective chemical denaturation using alkaline pH and detergent to separate plasmid…
Plasmid DNA Vs Chromosomal DNA
Criteria Plasmid DNA Chromosomal DNA Remarks Definition Small, circular, double-stranded extrachromosomal DNA molecule found mainly in bacteria and some eukaryotes…
2µ Plasmid
The 2µ plasmid, also called 2-µm plasmid, is a naturally occurring, circular DNA molecule found in Saccharomyces cerevisiae (budding yeast). …
Ti Plasmid
The Ti plasmid, or tumor-inducing plasmid, is an extrachromosomal DNA molecule found naturally in Agrobacterium tumefaciens. This bacterium is infamous…
Natural Hosts of Plasmids Across Life Domains
Plasmids occur naturally in a variety of hosts, primarily bacteria but also archaea and eukaryotes. In bacteria, plasmids are often…
Chloramphenicol Amplification of Low-copy Number Plasmids
Low-copy number plasmid vectors such as those based on pMB1 or ColE1 can be amplified by adding chloramphenicol to the…
Plasmid: pBR322 (Cloning Vector)
Plasmid name pBR322 Application Cloning vector Replicon pMBI Host E. coli DH5α, and other related strains Plasmid size (kbp) 4.361…
Plasmid Isolation: Genomic DNA vs Plasmid DNA
One of the major challenges in plasmid isolation methods is the separation of plasmid DNA from genomic DNA. To better…
Plasmid: pSymA
Plasmid name pSymA Host Sinorhizobium meliloti Plasmid size(kbp) 1354.2 Plasmid geometry Circular Plasmid copy number 2–3
Plasmid: SCP1
Plasmid name SCP1 Host Streptomyces coelicolor Plasmid size(kbp) 350.0 Plasmid geometry Linear Plasmid copy number 4
Plasmid: F
Plasmid name F Host Escherichia coli Plasmid size(kbp) 99.2 Plasmid geometry Circular Plasmid copy number 1–2