- ATP-dependent ligase enzyme from Bacteriophage T4
- Catalyzes the formation of a phosphodiester bond between juxtaposed 5′ phosphate and 3′ hydroxyl termini in duplex DNA or RNA.
- It can repair single-strand nicks in duplex DNA, RNA, or DNA/RNA hybrids but do not show any activity on single-stranded DNA or RNA.
- Used for both cohesive end and blunt end ligation in molecular cloning
Suppliers:
From ThermoFisherScientific
Product details: T4 DNA Ligase (5 U/µL)
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From NEB
Product details: T4 DNA Ligase
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From TaKaRa
Product details: T4 DNA Ligase
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From Promega
Product details: T4 DNA Ligase
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From Sigma-Aldrich
Product details: DNA Ligase from T4-infected Escherichia coli
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