Category: Lab Notes

Passaging/Subculturing Methods for Cells

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Enzymatic treatments (e.g., Trypsin, Collagenase, Dispase) Mechanical means (Scraping by rubber policeman, Dislodging semi-adherent cells by vigorous shaking) Treatment with…

Cryopreservation of Cells

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Cryopreservation is an efficient way to preserve cells at ultra-low temperatures (below -135°C) which stops all physiological processes and biological aging. It is a routinely used technique in all cell culture laboratories.

Cell culture overview

Cell Culture

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Culturing cells from multicellular organisms especially from animals (mammals, amphibians, insects, birds etc) refers to cell culture or animal cell culture.

Protocol – Cryopreservation of Adherent Cell Culture

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Further reading: Freshney, R. I., 2010. Basic Cell Culture Protocols / Culture of Animal Cells: A Manual of Basic Technique…