Cell Line: 293-PERV-PK-CIRCE

Synonyms:
- 293-PERV-PK-CIRCE
Description:
- Parent cell line: 293 (also known as HEK-293)
- Infected with endogenous retrovirus of pigs, produced by the pig kidney cell line PK-15
Keywords:
Culture Type: Cell line
Origin/Source:
- Organism:
  - Species: Human (Homo sapiens)
  - Age: Fetus
  - Gender: Female
- Tissue of Origin: Embryonic Kidney
Cell line features:
- Morphology:
- Cell Type: Embryonic cell
- Growth Mode: Adherent aggregates
- Doubling time:
- Karyotype:
Applications:
- Useful to examine the host range of the pig retrovirus to assess the risks associated with porcine xenotransplantation
- Pig retrovirus studies
Handling and maintenance:
- Biosafety level: BSL 2 (It may differ in your country, follow the guidelines of your institute/country)
- GMO Status: GMO1
- Complete culture medium:
  - DMEM + 2mM Glutamine + 10% Heat-inactivated FBS
- Culture conditions:
  - Temperature: Humidified 37°C incubator
  - Atmosphere: 5% CO₂, 95% Air
- Subculturing:
  - Subculturing procedure: Mechanical agitation
  - Harvesting time: Subconfluent culture (≈ 80%)
  - Subcultivation Ratio: 1:6
  - Medium Renewal: 2 to 3 times per week
  - Seeding densities:
- Cryopreservation:
  - Freezing medium: Complete culture medium + 20% Heat-inactivated FBS + 10% (v/v) DMSO
  - Cryopreservation temperature: Liquid nitrogen gas phase or -150°C freezer

Availability:
- Culture Collections (UK Health Security Agency): Product link
- Sigma-Aldrich: Product link
- Krackeler Scientific: Product link
References:
- https://www.culturecollections.org.uk/nop/product/293-perv-pk-circe
- https://www.cellosaurus.org/CVCL_2284
- Patience et al., 1997. Infection of human cells by an endogenous retrovirus of pigs C. Nat Med. 3(3), 282-286. PMID-9055854; Full-Text Links: Nature (Download PDF)
Abbreviations:
- DMSO (Dimethyl sulfoxide)
- Fetal bovine Serum (FBS)
- DMEM (Dulbecco’s Modified Eagle Medium)

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