- Synonyms:
- HEK-293A
- HEK293A
- HEK 293A
- HEK293-A
- 293A
- Culture Type: Cell line
- Keywords: Human Embryonic Kidney Cell Line
- Description: Cells express E1 proteins (E1a and E1b) needed to make recombinant adenoviruses
- Source:
- Organism:
- Species: Human (Homo sapiens)
- Age: fetus
- Species: Human (Homo sapiens)
- Tissue: Kidney; Embryo
- Organism:
- Cell line features:
- Morphology: Epithelial-like
- Cell Type: Epithelial
- Growth mode: Adherent, Monolayer
- Life span: Infinite (continuous culture)
- Handling and maintenance:
- Biosafety level: BSL 1 (cytion) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: Eagle’s Minimum Essential Medium + 2 mM L-Glutamine + 1.5 g/L NaHCO3 + 10% FBS
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 95% Air, 5% CO2
- Temperature: Humidified 37°C incubator
- Subculturing:
- Cell dissociation procedure: Accutase
- Subcultivation Ratio: 1:3 to 1:5
- Medium Renewal: 2 to 3 times per week
- Seeding densities:
- Harvesting densities:
- Split time: Split sub-confluent cultures (80%)
- Cell dissociation procedure: Accutase
- Cryopreservation
- Freeze medium: CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) (cytion)
- Storage conditions: Vapor phase of liquid nitrogen
- Applications:
- Production of high-quality viral preparations
- Availability
- Cytion: Product link
- References
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