- Synonyms: 293TGPRT+R1
- Description:
- Original cell line: 293 (also known as HEK-293)
- Immediate parent: 293T
- Cells stably express
- wild-type HIV Gag-Pol
- HIV tat
- HIV rev
- extra wild-type HIV rev
- Keywords:
- Culture Type: Cell line
- Origin/Source:
- Organism:
- Species: Human (Homo sapiens)
- Age: Fetus
- Tissue of Origin: Embryonic Kidney
- Organism:
- Cell line features:
- Morphology:
- Cell Type: Epithelial
- Growth Mode: Adherent
- Morphology:
- Applications:
- Packaging cell line for HIV-based vectors
- Handling and maintenance:
- Biosafety level: BSL 2 (It may differ in your country, follow the guidelines of your institute/country)
- GMO Status: GMO class 1
- Complete culture medium:
- DMEM + 2 mM L-Glutamine + 10% FBS
- DMEM + 2 mM L-Glutamine + 10% FBS
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 10% CO₂, 90% Air (culturecollections)
- Subculturing:
- Subculturing method: Enzyme-based cell dissociation
- Cell dissociation procedure: 0.05% trypsin or trypsin/EDTA
- Harvesting time: 80% confluence culture
- Split Ratio: 1:4 to 1:10
- Seeding densities: 3 – 5 X 104 cell/cm2
- Medium Renewal:
- Subculturing method: Enzyme-based cell dissociation
- Cryopreservation
- Cryopreservation temperature: Liquid nitrogen gas phase or -150°C freezer
- Availability:
- Culture Collections (UK Health Security Agency): Product link
- References:
- https://www.culturecollections.org.uk/nop/product/293tgprtr1
- Ikeda et. al., 2003. Continuous high-titer HIV-1 vector production. Nat. Biotechnol. 21(5), 569-572. PMID-12679787; Full-Text Link: Nature
- Abbreviations
- DMEM (Dulbecco’s Modified Eagle’s Medium)
- FBS (Fetal Bovine Serum)
- GMO (Genetically Modified Organism)
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