- Synonyms:
- 293SF-3F6
- Description:
- Original line: 293 (also known as HEK-293)
- Parent cell line: 293S
- Adapted to suspension culture in a serum-free medium followed by two consecutive cloning by limit dilution
- Keywords:
- Culture Type: Cell line
- Origin/Source:
- Organism:
- Species: Human (Homo sapiens)
- Age: Fetus
- Tissue of Origin: Embryonic Kidney
- Organism:
- Cell line features:
- Morphology: Lymphocyte-like (single cells to small aggregates)
- Cell Type: Embryonic cell
- Growth Mode: Suspension
- Doubling time: ≈ 24 hrs
- Morphology: Lymphocyte-like (single cells to small aggregates)
- Applications:
- Bioproduction
- Can be used to produce E1-deleted adenoviral vectors
- Handling and maintenance:
- Biosafety level: BSL 2 (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium:
- HyCell TransFx-H + 4 mM L-glutamine + 0.2% poloxamer 188 (Corning #13-901-CI)
- HyCell TransFx-H + 4 mM L-glutamine + 0.2% poloxamer 188 (Corning #13-901-CI)
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO₂, 95% Air
- Subculturing:
- Subculturing procedure: Dilution
- Harvesting time: When culture reaches cell density ≈1.5 x 106 and 3.0 x 106 viable cells/mL
- Medium Renewal: 2 to 3 times per week
- Seeding densities: 4 x 105 and 5 x 105 cells/mL
- Subculturing procedure: Dilution
- Cryopreservation:
- Complete culture medium + 5% (v/v) DMSO
- Cryopreservation temperature: Liquid nitrogen gas phase or -150°C freezer
- Availability:
- American Type Culture Collection (ATCC): Product link
- References:
- Abbreviations
- DMSO (Dimethyl sulfoxide)
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