Cell Line: HEK293S GnTI^–

Synonyms: HEK293S GnTI^–
Description:
- Parent cell line: 293 (also known as HEK-293)
- Established by methanesulfonate mutagenesis followed by ricin selection
- Cells do not have N-acetyl-glucosaminyltransferase I (GnTI) activity and therefore lack complex N-glycans.
Keywords:
Culture Type: Cell line
Origin/Source:
- Organism:
  - Species: Human (Homo sapiens)
  - Age: Fetus
- Tissue of Origin: Embryonic Kidney
Cell line features:
- Morphology: Epithelial-like
- Cell Type: Epithelial
- Growth Mode: Adherent
Applications:
- Bioproduction
- Widely used to overexpress various mammalian membrane proteins
Handling and maintenance
- Biosafety level: BSL 2 (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium:
  - DMEM:F12 Medium + 10% FBS
- Culture conditions:
  - Temperature: Humidified 37°C incubator
  - Atmosphere: 5% CO₂, 95% Air
- Subculturing:
  - Cell dissociation procedure: 0.05% (w/v) Trypsin – 0.53 mM EDTA solution
  - Harvesting time: 80% confluence culture
  - Subcultivation Ratio: 1:5 to 1:20
  - Medium Renewal: Every 2 to 3 days
  - Seeding densities:
- Cryopreservation
  - Complete culture medium + 5% DMSO
  - Cryopreservation temperature: Liquid nitrogen gas phase or -150°C freezer

Availability:
- American Type Culture Collection (ATCC): Product link
References:
- https://www.atcc.org/products/crl-3022
- Reeves et al. 2002. Structure and function in rhodopsin: High-level expression of rhodopsin with restricted and homogeneous N-glycosylation by a tetracycline-inducible N-acetylglucosaminyltransferase I-negative HEK293S stable mammalian cell line. Proc. Natl. Acad. Sci. USA 99 (21), 13419-13424. PMID-12370423; Full-Text Links: PNAS, PMC129688
Abbreviations
- DMEM (Dulbecco’s Modified Eagle’s Medium)
- FBS (Fetal Bovine Serum)

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