- Synonyms: OAT1 HEK 293T/17
- Description:
- Parent cell line: 293 (also known as HEK-293)
- Immediate parent: HEK 293T/17
- More than 75% cells are positive for OAT1
- Keywords:
- Culture Type: Cell line
- Origin/Source:
- Organism:
- Species: Human (Homo sapiens)
- Age: Fetus
- Tissue of Origin: Embryonic Kidney
- Organism:
- Cell line features:
- Morphology: Epithelial-like
- Cell Type: Epithelial
- Growth Mode: Adherent
- Morphology: Epithelial-like
- Applications:
- Toxicity testing for OAT1 transporter
- Toxicity testing for OAT1 transporter
- Handling and maintenance
- Biosafety level: BSL 2 (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium:
- DMEM + 10% heat-inactivated FBS + 3µg/mL Blasticidin
- DMEM + 10% heat-inactivated FBS + 3µg/mL Blasticidin
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO₂, 95% Air
- Subculturing:
- Cell dissociation procedure: 0.05% (w/v) Trypsin
- Harvesting time: 80% confluence culture
- Subcultivation Ratio:
- Medium Renewal:
- Seeding densities: 5.0 x 103 to 8.0 x 103 viable cells/cm2
- Cell dissociation procedure: 0.05% (w/v) Trypsin
- Cryopreservation
- Serum Free Cell Freezing Media (ATCC 30-2600)
- Cryopreservation temperature: Liquid nitrogen gas phase or -150°C freezer
- Availability:
- American Type Culture Collection (ATCC): Product link
- References:
- Abbreviations
- DMEM (Dulbecco’s Modified Eagle’s Medium)
- FBS (Fetal Bovine Serum)
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