Cell Line: 293T

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Synonyms: 293tsA1609neo, 293T
Description:
- A derivative of HEK-293 cell line, carries a gene for the temperature-sensitive SV40 T-antigen mutant tsA1609
- Produces replication-competent T antigen in large amounts at 37°C
Keywords:
Culture Type: Cell line
Origin/Source:
- Organism:
  - Species: Human (Homo sapiens)
  - Age: Fetus
- Tissue of Origin: Embryonic Kidney
Cell line features:
- Morphology: Epithelial-like
- Cell Type: Epithelial
- Growth Mode: Adherent
Applications:
- 3D cell culture
- Vaccine development
- Production of retroviruses
Handling and maintenance
- Biosafety level: BSL 2 (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium:
  - DMEM (2 mM L-glutamine) + 10% FBS
- Culture conditions:
  - Temperature: Humidified 37°C incubator
  - Atmosphere: 5% CO₂, 95% Air
- Subculturing:
  - Cell dissociation procedure: 0.05% (w/v) Trypsin – 0.53 mM EDTA solution
  - Harvesting time: 80% confluence culture
  - Subcultivation Ratio: 1:3 to 1:8
  - Medium Renewal: Every 2 to 3 days
  - Seeding densities:
- Cryopreservation
  - Growth medium + 5% DMSO
  - Cryopreservation temperature: Liquid nitrogen gas phase or -150°C freezer

Availability:
- American Type Culture Collection (ATCC): Product link
References:
- https://www.atcc.org/products/crl-3216
- DuBridge et al. 1987. Analysis of mutation in human cells by using an Epstein-Barr virus shuttle system. Mol. Cell Biol. 7, 379-387. PubMed: 3031469; Full-Text Links: tandfonline, PMC
- Pear et al. 1993. Production of high-titer helper-free retroviruses by transient transfection. Proc. Natl. Acad. Sci. USA. 90: 8392-8396. PubMed: 7690960; Full-Text Links: PNAS, PMC
Abbreviations
- DMEM (Dulbecco’s Modified Eagle’s Medium)
- FBS (Fetal Bovine Serum)

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