- Synonyms:
- 293T/17
- HEK293T/17
- HEK 293T/17
- HEK-293T/17
- 293T clone 17
- Description:
- Keywords:
- Culture Type: Cell line
- Origin/Source:
- Organism:
- Species: Human (Homo sapiens)
- Age: Fetus
- Tissue of Origin: Embryonic Kidney
- Organism:
- Cell line features:
- Morphology: Epithelial-like
- Cell Type: Epithelial
- Growth Mode: Adherent
- Morphology: Epithelial-like
- Applications:
- 3D cell culture
- Vaccine development
- Production of retroviruses
- Handling and maintenance
- Biosafety level: BSL 2 (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium:
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO₂, 95% Air
- Subculturing:
- Cryopreservation
- Growth medium + 5% DMSO
- Cryopreservation temperature: Liquid nitrogen gas phase or -150°C freezer
- Availability:
- American Type Culture Collection (ATCC): Product link
- Cytion: Product link
- References:
- https://www.atcc.org/products/crl-11268
- https://www.cytion.com/HEK293T-17/305117
- Sena-Esteves et al., 1999. Single-step conversion of cells to retrovirus vector producers with herpes simplex virus-Epstein-Barr virus hybrid amplicons. J. Virol. 73, 10426-10439. PMID-10559361; Full-Text Links: ASM, PMC
- Abbreviations
- DMEM (Dulbecco’s Modified Eagle’s Medium)
- FBS (Fetal Bovine Serum)
Was this post helpful?
Let us know if you liked the post. That’s the only way we can improve.
