Overview | |
Organism | Sweetwater fish – Poeciliid (Xiphophorus xiphidium), Strain: YF1 |
Source/Tissue | Embryonal, Rio Purificacion |
Morphology | Epithelial-like |
Cell type | Embryonal cells |
Growth mode | Loosely adherent, monolayer |
Doubling time | 2 – 3 days |
Life span | ? |
Karyotype | ? |
Transformed | ? |
Tumorigenic | ? |
Drug resistance | ? |
Biosafety Level | 1 REF |
Gene Expression | ? |
Products | ? |
Applications | ? |
Remarks | ? |
Related cell lines |
Cell line isolation information
The cell line A-2 was established from epithelial-like embryonal cells of the fish Xiphophorus xiphidium.
Recommended propagation method
Culture medium: DMEM : Ham’s F12 medium (1 : 1) + 15 – 20% heat-inactivated FBS
Culture conditions: Humidified incubator with 10% CO2 at 26 – 28°C
Split ratio: 1:3
Split frequency: 2-3 times per week
Harvesting time: Subconfluent culture (maximal density of 6-10 x 106 cells/80 cm2 )
Split Method: Shaking or pipetting
Recommended Cryopreservation Method
Freeze medium: 70% culture medium + 20% FBS + 10% DMSO
Storage temperature: Liquid nitrogen vapor phase
Availability
> Common Access to Biological Resources and Information (CABRI) : Catalog No. : ACC 276
> DSMZ : Catalog No. : ACC 276
References
> Common Access to Biological Resources and Information (CABRI)
> DSMZ