Cell Line : A-2

Overview 
OrganismSweetwater fish – Poeciliid (Xiphophorus xiphidium), Strain: YF1
Source/TissueEmbryonal, Rio Purificacion
MorphologyEpithelial-like
Cell typeEmbryonal cells
Growth modeLoosely adherent, monolayer
Doubling time2 – 3 days
Life span?
Karyotype?
Transformed?
Tumorigenic?
Drug resistance?
Biosafety Level1 REF
Gene Expression?
Products?
Applications?
Remarks?
Related cell lines 

Cell line isolation information

The cell line A-2 was established from epithelial-like embryonal cells of the fish Xiphophorus xiphidium.

Recommended propagation method

Culture medium: DMEM : Ham’s F12 medium (1 : 1) + 15 – 20% heat-inactivated FBS
Culture conditions: Humidified incubator with 10% CO2 at 26 – 28°C
Split ratio: 1:3
Split frequency: 2-3 times per week
Harvesting time: Subconfluent culture (maximal density of 6-10 x 106 cells/80 cm2 )
Split Method: Shaking or pipetting

Recommended Cryopreservation Method

Freeze medium: 70% culture medium + 20% FBS + 10% DMSO
Storage temperature: Liquid nitrogen vapor phase

Availability
> Common Access to Biological Resources and Information (CABRI) : Catalog No. : ACC 276
> DSMZ : Catalog No. : ACC 276

References

> Common Access to Biological Resources and Information (CABRI)
>
DSMZ

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