Cell Line : A 172

CELL LINE ORIGIN
OrganismHuman (Homo sapiens), 53 years old, Male
Source/TissueBrain, Glioblastoma
Year of origin?
CELL LINE FEATURES
MorphologyGlial-like
Cell type?
Growth modeAdherent, Monolayer
Doubling time?
Life spanInfinite (continuous culture)
Transformed?
TumorigenicNon-tumorigenic in immunosuppressed mice
Drug resistance?
Products?
KaryotypeHypertriploid
  
GENE EXPRESSION
  
APPLICATIONS
  
CULTURE MAINTENANCE AND STORAGE
Culture conditions and handling
Biosafety Level1 REF (It may differ in your country, follow the guidelines of your institute/country)
Plating efficiency?
Temperature37°C
AtmosphereHumidified incubator, 95% air + 5% CO2
Base mediumDMEM
Complete culture mediumDMEM (2mM Glutamine) + 10% FBS
Medium RenewalEvery 2-3 days
Subculturing procedure
Harvesting timeSubconfluent culture (70 – 80%)
Split methodTrypsin or Trypsin/EDTA
Split ratio1:3 to 1:8
Seeding density2 – 4 x 104 cells/cm²
Split frequencyEvery second or third day
Cryopreservation
Cryomedium

Complete culture medium + 5% DMSO
OR

70% complete culture medium + 20% FBS * + 10% DMSO (* One can increase FBS up to 50%)

Storage conditions
Liquid nitrogen vapor phase
AVAILABILITY / SOURCE

American Type Culture Collection (ATCC)

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Public Health England Culture Collections

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Sigma-Aldrich

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Japanese Collection of Research Bioresources (JCRB)

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Remarks 
Related cell lines 

 

References

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