Cell Line : A 172

Overview 
OrganismHuman (Homo sapiens), 53 years, Adult, Male
Source/TissueBrain, Glioblastoma
MorphologyGlial-like
Cell type?
Growth modeAdherent, Monolayer
Doubling time?
Life spanInfinite (continuous culture)
KaryotypeHypertriploid
Transformed?
TumorigenicNon-tumorigenic in immunosuppressed mice
Drug resistance?
Biosafety Level1 REF
Gene Expression?
Products 
Applications 
Remarks 
Related cell lines 

Cell line isolation information

The cell line A 172 was established from a glioblastoma tumor taken from a 53-year-old man.

Recommended propagation method

Culture medium: DMEM (+ 2mM Glutamine) + 10% FBS
Culture conditions: Humidified incubator, 95% air + 5% CO2, 37°C
Split ratio: 1:5 to 1:8
Split frequency: Every second or third day (twice or thrice a week)
Harvesting time: Subconfluent culture (≈ 80% )
Split Method: Trypsin/EDTA

Recommended Cryopreservation Method

Freeze medium: 70% complete culture medium + 20% FBS * + 10% DMSO
* One can increase the percentage of FBS up to 50%.
Storage temperature: Liquid nitrogen vapor phase

Availability
> American Type Culture Collection (ATCC) : Catalog No. : CRL-1620TM 
> Public Health England Culture Collections: Catalogue No.: 88062428
> Sigma-Aldrich: Catalog No.: 88062428

References

> Giard et al., 1973. In Vitro Cultivation of Human Tumors: Establishment of Cell Lines Derived From a Series of Solid Tumors. J Natl Cancer Inst. 51 (5), 1417-23. PMID-4357758 | Full Text – Oxford University Press

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