CELL LINE ORIGIN | |
Organism | Human (Homo sapiens), 53 years old, Male |
Source/Tissue | Brain, Glioblastoma |
Year of origin | ? |
CELL LINE FEATURES | |
Morphology | Glial-like |
Cell type | ? |
Growth mode | Adherent, Monolayer |
Doubling time | ? |
Life span | Infinite (continuous culture) |
Transformed | ? |
Tumorigenic | Non-tumorigenic in immunosuppressed mice |
Drug resistance | ? |
Products | ? |
Karyotype | Hypertriploid |
GENE EXPRESSION | |
APPLICATIONS | |
CULTURE MAINTENANCE AND STORAGE | |
Culture conditions and handling | |
Biosafety Level | 1 REF (It may differ in your country, follow the guidelines of your institute/country) |
Plating efficiency | ? |
Temperature | 37°C |
Atmosphere | Humidified incubator, 95% air + 5% CO2 |
Base medium | DMEM |
Complete culture medium | DMEM (2mM Glutamine) + 10% FBS |
Medium Renewal | Every 2-3 days |
Subculturing procedure | |
Harvesting time | Subconfluent culture (70 – 80%) |
Split method | Trypsin or Trypsin/EDTA |
Split ratio | 1:3 to 1:8 |
Seeding density | 2 – 4 x 104 cells/cm² |
Split frequency | Every second or third day |
Cryopreservation | |
Cryomedium | Complete culture medium + 5% DMSO |
Storage conditions | Liquid nitrogen vapor phase |
AVAILABILITY / SOURCE | |
American Type Culture Collection (ATCC) | Product page link |
Public Health England Culture Collections | Product page link |
Sigma-Aldrich | Product page link |
Japanese Collection of Research Bioresources (JCRB) | Product page link |
Remarks | |
Related cell lines |
References
- Giard et al., 1973. In Vitro Cultivation of Human Tumors: Establishment of Cell Lines Derived From a Series of Solid Tumors. J Natl Cancer Inst. 51 (5), 1417-23. PMID-4357758 | Full Text – Oxford University Press
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