| Overview | |
| Organism | Human (Homo sapiens), 53 years, Adult, Male |
| Source/Tissue | Brain, Glioblastoma |
| Morphology | Glial-like |
| Cell type | ? |
| Growth mode | Adherent, Monolayer |
| Doubling time | ? |
| Life span | Infinite (continuous culture) |
| Karyotype | Hypertriploid |
| Transformed | ? |
| Tumorigenic | Non-tumorigenic in immunosuppressed mice |
| Drug resistance | ? |
| Biosafety Level | 1 REF |
| Gene Expression | ? |
| Products | |
| Applications | |
| Remarks | |
| Related cell lines |
Cell line isolation information
The cell line A 172 was established from a glioblastoma tumor taken from a 53-year-old man.
Recommended propagation method
Culture medium: DMEM (+ 2mM Glutamine) + 10% FBS
Culture conditions: Humidified incubator, 95% air + 5% CO2, 37°C
Split ratio: 1:5 to 1:8
Split frequency: Every second or third day (twice or thrice a week)
Harvesting time: Subconfluent culture (≈ 80% )
Split Method: Trypsin/EDTA
Recommended Cryopreservation Method
Freeze medium: 70% complete culture medium + 20% FBS * + 10% DMSO
* One can increase the percentage of FBS up to 50%.
Storage temperature: Liquid nitrogen vapor phase
Availability
> American Type Culture Collection (ATCC) : Catalog No. : CRL-1620TM
> Public Health England Culture Collections: Catalogue No.: 88062428
> Sigma-Aldrich: Catalog No.: 88062428
References
> Giard et al., 1973. In Vitro Cultivation of Human Tumors: Establishment of Cell Lines Derived From a Series of Solid Tumors. J Natl Cancer Inst. 51 (5), 1417-23. PMID-4357758 | Full Text – Oxford University Press
