Cell Line: A-253

CELL LINE ORIGIN
OrganismHuman (Homo sapiens), 54 years adult caucasian male
Source/TissueSubmaxillary salivary gland, epidermoid carcinoma
Year of origin?
CELL LINE FEATURES
MorphologyEpithelial-like
Cell type?
Growth modeAdherent, Monolayer
Doubling time?
Life spanInfinite (continuous culture)
Transformed?
TumorigenicYes (in nude mice)
Drug resistance?
Products?
KaryotypeNear triploid
  
GENE EXPRESSION
  
APPLICATIONS
  
CULTURE MAINTENANCE AND STORAGE
Culture conditions and handling
Biosafety Level1 REF (It may differ in your country, follow the guidelines of your institute/country)
Plating efficiency?
Temperature37°C
AtmosphereHumidified incubator, 95% air + 5% CO2
Base mediumMcCoy’s 5a Medium Modified
Complete culture mediumMcCoy’s 5a Medium Modified + 10% FBS
Medium Renewal?
Subculturing procedure
Harvesting time?
Split methodTrypsin/EDTA
Split ratio1:2 to 1:4
Seeding density?
Split frequency?
Cryopreservation
CryomediumComplete culture medium + 5% DMSO
Storage conditions
Liquid nitrogen vapor phase
AVAILABILITY / SOURCE

American Type Culture Collection (ATCC)

Product page link
Remarks 
Related cell lines 

 

References

  • https://www.atcc.org/products/htb-41

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