Cell Line: A-375

  • Cell Line: A-375
  • Synonyms:
    • A-375
    • A375
    • A 375
    • A375-MEL
    • A375-mel
    • A375mel
  • Culture Type: Cell line
  • Keywords:  Human Melanoma Cell Line
  • Description: Established from skin tissue derived from a 54-year-old, Caucasian, female patient with malignant melanoma
  • Origin:
    • Organism:
      • Species: Human (Homo sapiens)
      • Ethnicity: Caucasian (Cellosaurus)
      • Age: 54 years
      • Gender: Female
    • Tissue: Skin
    • Disease: Malignant Melanoma
    • Primary/Metastasis: Primary (DepMap)
    • Collection site: Skin (DepMap)
    • Year of origin:-
    • Country of Origin: United States
  • Cell line features:
    • Cell Type: Epithelial
    • Morphology: Epithelial-like
    • Growth mode: Adherent
    • Life span: Infinite (continuous culture)
    • Doubling time: ~32 hours (Cellosaurus)
    • Karyotype: Hypotriploid, modal chromosome number: 62
    • Microsatellite instability: Stable
    • Special features:
    • Product:-
    • Soft agar colony formation assay:-
    • Tumorigenicity: Form tumors in immunosuppressed mice
    • Gene expression:-
    • Sequence variations:
      • BRAF (V600E mutation, Oncogenic, Gain-of-function, Homozygous)
      • For more information, please visit:
        • https://depmap.org/portal/cell_line/ACH-000219?tab=mutations
        • https://depmap.org/portal/cell_line/ACH-000219?tab=fusions
  • Handling and maintenance:
    • Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
    • Complete culture medium: Dulbecco’s Modified Eagle’s Medium + 10%  FBS (ATCC)
    • Culture conditions: 
      • Temperature: 37°C incubator
      • Atmosphere: 95% Air, 5% CO2
    • Subculturing:
      • Subculturing method: Enzymatic dissociation
      • Cell dissociation solution: Trypsin-EDTA solution
      • Harvesting time: sub-confluent culture
      • Seeding density:-
      • Split ratio: 1:3 to 1:8
      • Split frequency:-
      • Medium Renewal:  Every 2 to 3 days
    • Cryopreservation:
      • Freezing medium: Complete growth medium + 5% (v/v) DMSO (ATCC)
      • Storage conditions: Vapor phase of liquid nitrogen
      • Cell suspension (cell density):-
      • Volume per vial: 1 ml
  • Applications: 
    • Cancer research
    • 3D cell culture
    • High-throughput screening
    • Toxicology
  • Cell Line Availability: The cell line can be obtained from the following sources.

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