- Cell Line: A-375
- Synonyms:
- A-375
- A375
- A 375
- A375-MEL
- A375-mel
- A375mel
- Culture Type: Cell line
- Keywords: Human Melanoma Cell Line
- Description: Established from skin tissue derived from a 54-year-old, Caucasian, female patient with malignant melanoma
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Ethnicity: Caucasian (Cellosaurus)
- Age: 54 years
- Gender: Female
- Species: Human (Homo sapiens)
- Tissue: Skin
- Disease: Malignant Melanoma
- Primary/Metastasis: Primary (DepMap)
- Collection site: Skin (DepMap)
- Year of origin:-
- Country of Origin: United States
- Organism:
- Cell line features:
- Cell Type: Epithelial
- Morphology: Epithelial-like
- Growth mode: Adherent
- Life span: Infinite (continuous culture)
- Doubling time: ~32 hours (Cellosaurus)
- Karyotype: Hypotriploid, modal chromosome number: 62
- Microsatellite instability: Stable
- Special features:
- Product:-
- Soft agar colony formation assay:-
- Tumorigenicity: Form tumors in immunosuppressed mice
- Gene expression:-
- Sequence variations:
- BRAF (V600E mutation, Oncogenic, Gain-of-function, Homozygous)
- For more information, please visit:
- https://depmap.org/portal/cell_line/ACH-000219?tab=mutations
- https://depmap.org/portal/cell_line/ACH-000219?tab=fusions
- Cell Type: Epithelial
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: Dulbecco’s Modified Eagle’s Medium + 10% FBS (ATCC)
- Culture conditions:
- Temperature: 37°C incubator
- Atmosphere: 95% Air, 5% CO2
- Temperature: 37°C incubator
- Subculturing:
- Subculturing method: Enzymatic dissociation
- Cell dissociation solution: Trypsin-EDTA solution
- Harvesting time: sub-confluent culture
- Seeding density:-
- Split ratio: 1:3 to 1:8
- Split frequency:-
- Medium Renewal: Every 2 to 3 days
- Subculturing method: Enzymatic dissociation
- Cryopreservation:
- Freezing medium: Complete growth medium + 5% (v/v) DMSO (ATCC)
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial: 1 ml
- Freezing medium: Complete growth medium + 5% (v/v) DMSO (ATCC)
- Applications:
- Cancer research
- 3D cell culture
- High-throughput screening
- Toxicology
- Cell Line Availability: The cell line can be obtained from the following sources.
- American Type Culture Collection (ATCC): Product link
- UK Health Security Agency Culture Collections: Product link
- SigmaAldrich: Product link
- Cyton: Product link
- References
- Images:
- A-375 cell line image link 1 (Source: ATCC)
- A-375 cell line image link 2 (Source: DepMap)
- A-375 cell line image link 3 (Source: Cytion)
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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