Cell Line: A-375

CELL LINE ORIGIN
OrganismHuman (Homo sapiens), 54 years old, female
Source/TissueSkin, malignant melanoma
Year of origin?
CELL LINE FEATURES
MorphologyEpithelial-like
Cell type?
Growth modeAdherent, Monolayer
Doubling time?
Life spanInfinite (continuous culture)
Transformed?
TumorigenicYes, in immunosuppressed mice
Drug resistance?
Products?
KaryotypeHypotriploid
  
GENE EXPRESSION
  
APPLICATIONS
 Suitable transfection host
CULTURE MAINTENANCE AND STORAGE
Culture conditions and handling
Biosafety Level1 REF / 2 REF(It may differ in your country, follow the guidelines of your institute/country)
Plating efficiency?
Temperature37°C
AtmosphereHumidified incubator, 95% air + 5% CO2
Base mediumDMEM
Complete culture mediumDMEM (2mM Glutamine) + 10% FBS
Medium RenewalEvery 2 to 3 days
Subculturing procedure
Harvesting timeSubconfluent culture
Split methodTrypsin or Trypsin/EDTA
Split ratio1:3 to 1:8
Seeding density1 – 3 x 104 cells/cm²
Split frequencyEvery second or third day
Cryopreservation
CryomediumComplete culture medium + 5% DMSO
Storage conditions
Liquid nitrogen vapor phase
AVAILABILITY / SOURCE

American Type Culture Collection (ATCC)

Product page link

Public Health England Culture Collections

Product page link

Sigma-Aldrich

Product page link
Remarks 
Related cell lines 

 

References

  • https://www.atcc.org/products/crl-1619
  • https://www.culturecollections.org.uk/products/celllines/generalcell/detail.jsp?refId=88113005&collection=ecacc_gc

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