Cell Line: MKN1

OVERVIEW

 
OrganismHuman (Homo sapiens), 72 years old Japanese male (Ref: riken)
Source/Tissue of origin
Stomach, Lymph node metastasis (Ref: riken)
Year of isolation 1975 (Ref: riken)
MorphologyEpithelial-like (Ref: riken)
Cell type 
Growth modeAdherent, monolayer (Ref: riken)
Doubling time
 
Life spanInfinite, continuous culture
Karyotype 
Transformed 
Tumorigenic 
Drug resistance 
Biosafety LevelBSL 1 (Ref: riken) (It may differ in your country, follow the guidelines of your institute/country)
Gene Expression 
Products 
Applications 
Remarks 
KeywordsA human gastric epithelial cancer cell line
Related cell lines 

Culture maintenance

Culture medium*
3. RPMI 1640 + 10% FBS
Culture conditionsHumidified incubator, 95% air + 5% CO2, 37°C
Subculturing procedureTrypsin-EDTA

Culture storage

Cryo-medium
Complete growth medium + 5% (v/v) (Ref: atcc)
Storage conditions
Vapor phase of liquid nitrogen (Ref: atcc)

REFERENCES

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