- Synonyms: 293-Hektor
- Keywords: Human embryo kidney cell line
- Description:
- Parent cell line: 293 (also known as HEK-293)
- HEK293 cells are adapted to grow in suspension in the chemically defined protein- and peptide-free Hektor G medium
- Culture Type: Cell line
- Origin/Source:
- Organism:
- Species: Human
- Age: Fetus
- Tissue of Origin: Embryonic Kidney
- Organism:
- Cell line features:
- Morphology: Small slightly rounded cells growing in a dispersed manner
- Cell Type: Fibroblastic (culturecollections)
- Growth Mode: Aggregates in suspension, some cells adherent
- Morphology: Small slightly rounded cells growing in a dispersed manner
- Applications:
- Handling and maintenance:
- Biosafety level: BSL 2 (It may differ in your country, follow the guidelines of your institute/country)
- GMO Status: GMO1
- Complete culture medium:
- Hektor G medium + 4mM Glutamine + 5 µg/ml Insulin
- Hektor G medium + 4mM Glutamine + 5 µg/ml Insulin
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: ?
- Subculturing:
- Cell dissociation procedure: Dilution
- Harvesting time:
- Subcultivation Ratio:
- Medium Renewal:
- Seeding densities: ?
- Cell dissociation procedure: Dilution
- Cryopreservation
- 50:50 conditioned medium:fresh medium + 10% DMSO
- Cryopreservation temperature: Liquid nitrogen gas phase or -150°C freezer
- Availability:
- Culture Collections (UK Health Security Agency): Product link
- CellBank Australia: Product Link
- References:
- Abbreviations
- GMO1 (Genetically Modified Organism Class 1)
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