- Synonyms
- K-562-GFP
- K562-GFP
- Culture Type: Cell line
- Keywords: GFP-expressing human chronic myeloid leukemia in blast crisis
- Source: K562-GFP is a GFP-expressing subclone of K562 cell line. These cells stably express GFP under the control of the human EF-1 alpha promoter.
- Organism: Human (Homo sapiens), 53-year-old caucasian female
- Tissue of Origin: blood
- Tissue: Bone; Marrow, pleural effusion
- Disease: Chronic myeloid leukemia in blast crisis
- Cell line features:
- Morphology: round and large, single cells
- Cell Type: Lymphoblast
- Growth Mode: Suspension
- Population doubling time: ≈ 16 – 20 h
- Life span: Infinite, continuous culture
- Cytogenetics:
- Human hypotriploid karyotype
- Handling
- Biosafety Information: BSL 2 (ATCC)
(It may differ in your country, follow the guidelines of your institute/country) - Complete culture medium:
- RPMI 1640 (2 mM Glutamine) + 10% FBS + 0.5 µg/ml puromycin
- Culture conditions
- Temperature: 37°C
- Atmosphere: 95% Air, 5% CO2
- Subculturing procedure: Dilution (replacement or addition of fresh culture medium)
- Maintain cultures between 1 x 105 viable cells/mL to 1 x 106 cells/mL
- Medium Renewal: Every 2 to 3 days
- Split ratio: 1:3 to 1:5 every 3 days
- Cryopreservation
- Storage conditions: Vapor phase of liquid nitrogen
- Freezing medium: Complete growth medium + 5% (v/v) DMSO (without puromycin)
- Cells per vial: 1 ml of cell suspension containing 2- 3 x 106 cells
- Biosafety Information: BSL 2 (ATCC)
- Availability
- ATCC CCL-243-GFP ™
- Applications:
- These cells enable highly accurate quantitation of killing by flow cytometry in the natural killer assay.
- References:
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