- Synonyms
- K-562 cl.6
- K562 cl.6
- Culture Type: Cell line
- Keywords: Human chronic myeloid leukemia in blast crisis
- Source: K-562 cl.6 is a subclone of K562 cell line.
- Organism: Human (Homo sapiens), 53 year-old caucasian female
- Tissue of Origin: blood
- Tissue: Bone; Marrow, pleural effusion
- Disease: Chronic myeloid leukemia in blast crisis
- Cell line features:
- Morphology: round and large, single cells
- Cell Type: Lymphoblast
- Growth Mode: Suspension
- Life span: Infinite, continuous culture
- Cytogenetics:
- Human hypotriploid karyotype
- Handling
- Biosafety Information: BSL 2 (ECACC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium:
- RPMI 1640 (2mM Glutamine) + 10% FBS
- Culture conditions
- Temperature: 37°C
- Atmosphere: 95% Air, 5% CO2
- Subculturing procedure: Dilution (addition or replacement of fresh culture medium)
- Maintain cultures between 2 x 105 viable cells/mL to 1 x 106 cells/mL
- Medium Renewal: Every 2 to 3 days
- Split ratio: 1:3 to 1:5 every 3 days
- Cryopreservation
- Storage conditions: Vapor phase of liquid nitrogen
- Freezing medium: Complete growth medium + 5% (v/v) DMSO
- Applications:
- Highly sensitive in vitro target for the natural killer assay to assess human natural killer activity
- References:
- European Collection of Authenticated Cell Cultures (ECACC)
- Alder et al., 1984. A kinetic and clonal analysis of heterogeneity in K562 cells. J Cell Physiol. 118(2), 186-92. PMID-6693507; Full-Text Link: Wiley
- Renshaw et al., 2004. Disruption of WT1 gene expression and exon 5 splicing following cytotoxic drug treatment: antisense down-regulation of exon 5 alters target gene expression and inhibits cell survival. Mol Cancer Ther. 3(11), 1467-1484. PMID-15542786
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