- Cell Line: NCI-H82
- Synonyms:
- NCI-H82
- NCIH82
- H82
- H-82
- Culture Type: Cell line
- Keywords: Human Small Cell Lung Carcinoma Epithelial Cell Line
- Description: Established from the Pleural effusion (metastatic site) of a male 40/41-year-old Caucasian patient with Small Cell Lung Carcinoma (Neuroendocrine Tumor)
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age: 40/41 years
- Ethnicity: Caucasian
- Gender: Male
- Tissue (origin): Lung
- Tissue (isolation): Pleural effusion (metastatic site)
- Disease:
- Small Cell Lung Carcinoma/Lung Neuroendocrine Tumor
- Primary/Metastasis: Metastatic
- Small Cell Lung Carcinoma/Lung Neuroendocrine Tumor
- Year of origin: 1978
- Organism:
- Cell line features:
- Cell Type:-
- Morphology: Aggregates in suspension
- Growth mode: Suspension
- Life span: Infinite (continuous culture)
- Karyotype: Near triploid, Modal chromosome number: 58
- Doubling time: ≈30 hours
- Microsatellite instability: Stable
- Special features:-
- Product:-
- Tumorigenicity: Yes, in nude mice
- Gene expression:
- Stain positively for neurofilaments and vimentin
- Sequence variations:
- 25-fold amplification of C-myc DNA sequences and 24-fold increase in c-myc RNA relative to normal cells
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- Cell Type:-
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: RPMI-1640 Medium + 10% FBS
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Dilution
- Harvesting time:-
- Split ratio: 1:2 to 1:5
- Medium Renewal: 2 to 3 times per week
- Cryopreservation:
- Freezing medium: Complete growth medium + 5% DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial:-
- Applications:
- Cancer research
- 3D cell culture
- Availability
- American Type Culture Collection (ATCC): Product link
- DSMZ-German Collection of Microorganisms and Cell Cultures GmbH: Product link
- AcceGen: Product link
- Cytion: Product link
- References
- Images:
- NCI-H82 cell image link (Source: DSMZ)
- NCI-H82 cell image link (Source: DSMZ)
- NCI-H82 cell image link (Source: DSMZ)
- NCI-H82 cell image link (Source: DSMZ)
- NCI-H82 cell image link (Source: Cytion)
- NCI-H82 cell image link (Source: Cytion)
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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- Please let us know if you find any mistakes. Your comments are welcome in the comment box.
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